Construct: ORF TRCN0000476311
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011390.1_s317c1
- Derived from:
- ccsbBroadEn_07816
- DNA Barcode:
- ACCATGGCAAGAGGGTCGATAAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLRK1 (22914)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476311
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100528032 | KLRC4-KLRK1 | KLRC4-KLRK1 readthrough | NM_001199805.1 | 99.6% | 99.5% | 214A>G;624G>A |
| 2 | human | 22914 | KLRK1 | killer cell lectin like rec... | NM_007360.4 | 99.6% | 99.5% | 214A>G;624G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 717
- ORF length:
- 648
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggggtggatt cgtggtcgga ggtctcgaca cagctgggag atgagtgaat 121 ttcataatta taacttggat ctgaagaaga gtgatttttc aacacgatgg caaaagcaaa 181 gatgtccagt agtcaaaagc aaatgtagag aaaatgcatc tccatttttt ttctgctgct 241 tcatcgctgt agccatggga atccgtttca ttattatggt agcaatatgg agtgctgtat 301 tcctaaactc attattcaac caagaagttc aaattccctt gaccgaaagt tactgtggcc 361 catgtcctaa aaactggata tgttacaaaa ataactgcta ccaatTTTTT GATGAGAGTA 421 AAAACTGGTA TGAGAGCCAG GCTTCTTGTA TGTCTCAAAA TGCCAGCCTT CTGAAAGTAT 481 ACAGCAAAGA GGACCAGGAT TTACTTAAAC TGGTGAAGTC ATATCATTGG ATGGGACTAG 541 TACACATTCC AACAAATGGA TCTTGGCAGT GGGAAGATGG CTCCATTCTC TCACCCAACC 601 TACTAACAAT AATTGAAATG CAGAAGGGAG ACTGTGCACT CTATGCCTCG AGCTTTAAAG 661 GCTATATAGA AAACTGTTCA ACTCCAAATA CATACATCTG CATGCAAAGG ACTGTGTTGC 721 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 781 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 841 ATATATCTTG TGGAAAGGAC GAACCATGGC AAGAGGGTCG ATAAAAACGC GTTAAGTCga 901 caatcaacct ctggattaca aaatttgtga aagatt