Construct: ORF TRCN0000476326
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000591.1_s317c1
- Derived from:
- ccsbBroadEn_14473
- DNA Barcode:
- ATCGCCATAGCGCTCGGACTCGTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- LUZP2 (338645)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476326
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 338645 | LUZP2 | leucine zipper protein 2 | NM_001009909.4 | 99.6% | 6% | 60_63delCAGA |
| 2 | human | 338645 | LUZP2 | leucine zipper protein 2 | NM_001252010.2 | 87.4% | 6.9% | 60_63delCAGA;394_395ins126 |
| 3 | human | 338645 | LUZP2 | leucine zipper protein 2 | NM_001252008.2 | 75.4% | 2.6% | 0_1ins254 |
| 4 | human | 338645 | LUZP2 | leucine zipper protein 2 | XM_024448468.1 | 73% | 1.4% | 1_382del |
| 5 | human | 338645 | LUZP2 | leucine zipper protein 2 | XM_017017648.2 | 62.4% | 1.3% | (many diffs) |
| 6 | human | 338645 | LUZP2 | leucine zipper protein 2 | XM_017017649.2 | 37.6% | 1.2% | (many diffs) |
| 7 | human | 338645 | LUZP2 | leucine zipper protein 2 | XM_011520056.3 | 28.2% | 1.4% | (many diffs) |
| 8 | human | 338645 | LUZP2 | leucine zipper protein 2 | XR_930864.3 | 24% | 1_756del;1146_1320del;1404_1405ins561 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 144
- ORF length:
- 75
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaaattcagc ccagcgcact acctgctgcc tctcctgcct gcgctggtcc 121 tcagcaccag gactatgaag agctagaaaa gcagctgaaa gaagtcttta aggagcgaag 181 caccattctt cgtcagctga caaagacatc aagagaactt gatggaatta aagtcaatct 241 tcagtcctta aaaaacgatg agcagtctgc caaaactgat gttcagaaac ttctggaatt 301 aggacagaaa caaagagaag aaatgaagtc tcttcaggag gccctgcaaa atcagcttaa 361 ggagacatca gagaaagcag aaaaacacca ggctactatt aattttttaa agactgaagt 421 tgaaagaaag agcaaaatga tccgagacct ccagaatgag aataaaagct tgaaaaacaa 481 actcttgtca ggaaacaagc tctgtggcat tcacgcagaa gagtcaaaaa aaatccaagc 541 ccagctgaag gagcttcgtt atgggaagaa ggatttatta tttaaggcgc agcagcttac 601 tgatctggaa caaaaattag ctgtagccaa aaatgaactg gagaaagcag ctcttgacag 661 ggagtcacag atgaaagcaa tgaaagagac tgtgcagctc tgcttgacat ctgtttTCCG 721 TGATCAGCCT CCTCCCCCTT TGAGTTTAAT CACATCAAAT CCAACTCGGA TGTTACTCCC 781 ACCCAGGAAT ATTGCCTCTA AGCTTCCAGA TGCAGCGGCC AAAAGCAAGC CTCAACAAAG 841 TGCTTCTGGA AACAATGAGA GCTCTCAAGT TGAGTCAACA AAGGAAGGAA ATCCAAGTAC 901 CACTGCCTGT GACTCTCAAG ATGAGGGCAG ACCGTGTTCC ATGAAGCACA AAGAAAGTCC 961 CCCAAGTAAT GCCACTGCAG AAACCGAGCC TATCCCACAG AAATTGCAAA TGCCTCCTTG 1021 CTCTGAATGT GAGGTGAAAA AAGCCCCAGA AAAACCATTG ACCAGCTTTG AAGGGATGGC 1081 AGCTAGAGAA GAAAAAATAC TGTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA 1141 GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT 1201 TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAT CGCCATAGCG 1261 CTCGGACTCG TCACGCGTTA AGTCgacaat caacctctgg attacaaaat ttgtgaaaga 1321 tt