Construct: ORF TRCN0000476453
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009276.1_s317c1
- Derived from:
- ccsbBroadEn_13559
- DNA Barcode:
- GCTTCAAATACCACCTCGCAGGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATG9B (285973)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476453
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285973 | ATG9B | autophagy related 9B | NM_001317056.1 | 44.3% | 39.7% | 1_787del;963_1717del |
| 2 | human | 285973 | ATG9B | autophagy related 9B | XM_024446722.1 | 44.3% | 39.7% | 1_787del;963_1717del |
| 3 | human | 285973 | ATG9B | autophagy related 9B | XM_011516065.2 | 42.3% | 37.9% | 1_919del;1095_1849del |
| 4 | human | 285973 | ATG9B | autophagy related 9B | XM_011516066.3 | 42.3% | 37.9% | 1_919del;1095_1849del |
| 5 | human | 285973 | ATG9B | autophagy related 9B | XM_024446719.1 | 42.3% | 37.9% | 1_919del;1095_1849del |
| 6 | human | 285973 | ATG9B | autophagy related 9B | XM_024446720.1 | 42.3% | 37.9% | 1_919del;1095_1849del |
| 7 | human | 285973 | ATG9B | autophagy related 9B | XM_024446721.1 | 42.3% | 37.9% | 1_919del;1095_1849del |
| 8 | human | 285973 | ATG9B | autophagy related 9B | XR_002956421.1 | 33.5% | 1_1378del;1554_2308del;3364_3668del | |
| 9 | human | 285973 | ATG9B | autophagy related 9B | NR_073169.1 | 32% | 1_470del;1701_3840del | |
| 10 | human | 285973 | ATG9B | autophagy related 9B | NR_133652.1 | 26.8% | 1_863del;1039_1793del;2849_4577del | |
| 11 | mouse | 213948 | Atg9b | autophagy related 9B | NM_001002897.3 | 37.7% | 34.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1299
- ORF length:
- 1230
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gccatcctac cctcagccca gtgtgctgag aggatccgct ccagcccgct 121 gctggtcctc ctcctggtcc tggctgccgg cttctggctg gtccaactgc ttcgctcagt 181 ctgcaacctc ttcagctact gggacatcca ggtgttttac agggaggccc tgcacatccc 241 cccggtcttt cattccggaa gagcagtgcc agggtcgtgc gccgcagctc ctgctgcaga 301 cagccctggc ccacatgcac tacctcccgg aggagcccgg ccccggcggc agggaccgcg 361 cctaccggca gatggcgcag ctgctgcagt accgagcggt ctccctcctg gaggagctcc 421 tgtccccgct cctcaccccg ctgtttctgc ttttctggtt ccgccctcgt gccctggaga 481 ttatcgactt ttttcatcac ttcactgtgg atgtggctgg ggttggggac atctgttcct 541 ttgcccttat ggatgtgaag cgccacggac accctcagtg gctctcggcg ggacagactg 601 aggcctcgct gtctcagcgt gcggaggacg gcaagactga gctttctttg atgcggttct 661 ccctggcgca tccactctgg cgccccccag ggcacagctc taagtttctt gggcacctct 721 ggggccgagt acaacaagat gcagctgcct ggggtgccac ctcggctcgc ggcccctcca 781 ccccgggggt gctcagcaac tgcacctcgc ccctgcctga ggccttcctg gccaacctct 841 tcgtgcaccc tctcctgcct ccgagagatc tgagcccgac agccccctgt ccagctgcgg 901 ccacagccag cctccttgcc TCCATTTCCC GAATTGCCCA GGACCCCAGC TCTGTGTCCC 961 CAGGAGGCAC TGGGGGCCAG AAGCTGGCCC AGCTCCCAGA ACTTGCTTCT GCCGAGATGA 1021 GTCTCCATGT CATCTACCTG CACCAGCTTC ACCAGCAGCA GCAGCAGCAG GAGCCGTGGG 1081 GTGAGGCTGC AGCCTCCATC CTGTCCAGGC CCTGCTCCAG CCCCTCACAG CCACCCTCGC 1141 CTGATGAGGA GAAGCCATCC TGGTCAAGTG ACGGCTCCAG TCCTGCCTCT AGCCCCAGAC 1201 AACAGTGGGG AACCCAGAAG GCCCGGAATC TGTTCCCCGG AGGGTTTCAG GTGACCACAG 1261 ACACCCAGAA GGAGCCTGAC CGGGCCTCTT GCACTGACTT GCCAACTTTC TTGTACAAAG 1321 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1381 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1441 ACGAGCTTCA AATACCACCT CGCAGGTCAC GCGTTAAGTC gacaatcaac ctctggatta 1501 caaaatttgt gaaagatt