Construct: ORF TRCN0000476474
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012685.1_s317c1
- Derived from:
- ccsbBroadEn_06085
- DNA Barcode:
- TTAGAATCCCTTTACCCACTCCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DCK (1633)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476474
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1633 | DCK | deoxycytidine kinase | NM_000788.3 | 99.8% | 100% | 300C>T |
| 2 | mouse | 13178 | Dck | deoxycytidine kinase | NM_007832.4 | 88.2% | 93.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 849
- ORF length:
- 780
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccaccccg cccaagagaa gctgcccgtc tttctcagcc agctctgagg 121 ggacccgcat caagaaaatc tccatcgaag ggaacatcgc tgcagggaag tcaacatttg 181 tgaatatcct taaacaattg tgtgaagatt gggaagtggt tcctgaacct gttgccagat 241 ggtgcaatgt tcaaagtact caagatgaat ttgaggaact tacaatgtct cagaaaaatg 301 gtgggaatgt tcttcagatg atgtatgaga aacctgaacg atggtctttt accttccaaa 361 catatgcttg tctcagtcga ataagagctc agcttgcctc tctgaatggc aagctcaaag 421 atgcagagaa acctgtatta ttttttgaac gatctgtgta tagtgacagg tatatttttg 481 catctaattt gtatgaaTCT GAATGCATGA ATGAGACAGA GTGGACAATT TATCAAGACT 541 GGCATGACTG GATGAATAAC CAATTTGGCC AAAGCCTTGA ATTGGATGGA ATCATTTATC 601 TTCAAGCCAC TCCAGAGACA TGCTTACATA GAATATATTT ACGGGGAAGA AATGAAGAGC 661 AAGGCATTCC TCTTGAATAT TTAGAGAAGC TTCATTATAA ACATGAAAGC TGGCTCCTGC 721 ATAGGACACT GAAAACCAAC TTCGATTATC TTCAAGAGGT GCCTATCTTA ACACTGGATG 781 TTAATGAAGA CTTTAAAGAC AAATATGAAA GTCTGGTTGA AAAGGTCAAA GAGTTTTTGA 841 GTACTTTGTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 901 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 961 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATTAGAA TCCCTTTACC CACTCCCGAC 1021 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt