Construct: ORF TRCN0000476600
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009105.1_s317c1
- Derived from:
- ccsbBroadEn_14173
- DNA Barcode:
- ACTTTCGTCGTGGGTACCGCTATC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALKBH5 (54890)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476600
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54890 | ALKBH5 | alkB homolog 5, RNA demethy... | NM_017758.4 | 84.4% | 84% | (many diffs) |
| 2 | mouse | 268420 | Alkbh5 | alkB homolog 5, RNA demethy... | NM_172943.4 | 76.3% | 81.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1440
- ORF length:
- 1374
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggn ggccgccagc ggctacacgg acctgcgtga gaagctcaag tccatgacgt 121 cccgggacaa ctataaggcg ggcagccggg aggccgccgc cgctgccgca gccgccgtag 181 ccgccgcagc cgcagccgcc gctgccgccg aaccttaccc tgtgtccggg gccaagcgca 241 agtatcagga ggactcggac cccgagcgca gcgactatga ggagcagcag ctgcagaagg 301 aggaggaggc gcgcaaggtg aagagcggca tccgccagat gcgcctcttc agccaggacg 361 agtgcgccaa gatcgaggcc cgcattgacg aggtggtgtc ccgcgctgag aagggcctgt 421 acaacgagca cacggtggac cgggccccac tgcgcaacaa gtacttcttc ggcgaaggct 481 acacttacgg cgcccagctg cagaagcgcg ggcccggcca ggagcgcctc tacccgccgg 541 gcgacgtgga cgagatcccc gagtgggtgc accagctggt gatccaaaag ctggtggagc 601 accgcgtcat ccccgagggc ttcgtcaaca gcgccgtcat caacgactac cagcccggcg 661 gctgcatcgt gtctcacgtg gaccccatcc acatcttcga gcgccccatc gtgtccgtgt 721 ccttctttag cgactctgcg ctgtgcttcg gctgcaagtt ccagttcaag cctattcggg 781 tgtcggaacc agtgctttcc ctgccggtgc gcaggggaag cgtgactgtg ctcagtggat 841 atgctgctga tgaaatcact cactgcatac ggcctcagga catcaaggag cgccgagcag 901 tcatcatcct caggaagaca agattagatg caccccggtt ggaaacaaag tccctgagca 961 gctccgtgtt accacccagc tatgcttcag atcgcctgtc aggaaacaac agggaccctg 1021 ctctgaaacc caAGCGGTCC CACCGCAAGG CAGACCCTGA TGCTGCCCAC AGGCCACGGA 1081 TTCTGGAGAT GGACAAGGAA GAGAACCGGC GCTCGGTGCT GCTGCCCACA CACCGGCGGA 1141 GGGGTAGCTT CAGCTCTGAG AACTACTGGC GCAAGTCATA CGAGTCCTCA GAGGACTGCT 1201 CTGAGGCAGC AGGCAGCCCT GGGAACTCTG GCTCATCCTT ACGTAGTTGC CCCTCCTTTT 1261 GTTTTGAGGG TTTTGTTTTT GTTCATTGGG GGGTTTTTGT TTTTTGTTTT TTGTTTTTTT 1321 TGATTCTATA TATTTTTCCT TGGTTTTGTT GCCTGTTAGG GCTGAAGAAT AGAATTGGCC 1381 AGGACCTAGG TTCTCATATT CTTGGTATTC CTCCTGGATG GAAAGGCTTT GGCATCAATA 1441 CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG 1501 TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT 1561 TTATATATCT TGTGGAAAGG ACGAACTTTC GTCGTGGGTA CCGCTATCAC GCGTTAAGTC 1621 gacaatcaac ctctggatta caaaatttgt gaaagatt