Construct: ORF TRCN0000476602
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003313.1_s317c1
- Derived from:
- ccsbBroadEn_08094
- DNA Barcode:
- AGCTTAGCACTATAGGTAGCTATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OSTM1 (28962)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476602
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 28962 | OSTM1 | osteoclastogenesis associat... | NM_014028.4 | 99.9% | 100% | 924G>A |
| 2 | human | 28962 | OSTM1 | osteoclastogenesis associat... | XR_942410.3 | 32.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1071
- ORF length:
- 1002
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagccgggc ccgacagccg cgcagcggag gtgttcgttg ccgccgtggc 121 tgccgctggg gctgctgctg tggtcggggc tggccctggg cgcgctcccc ttcggcagca 181 gtccgcacag ggtcttccac gacctcctgt cggagcagca gttgctggag gtggaggact 241 tgtccctgtc cctcctgcag ggtggagggc tggggcctct gtcgctgccc ccggacctgc 301 cggatctgga tcctgagtgc cgggagctcc tgctggactt cgccaacagc agcgcagagc 361 tgacagggtg tctggtgcgc agcgcccggc ccgtgcgcct ctgtcagacc tgctaccccc 421 tcttccaaca ggtcgtcagc aagatggaca acatcagccg agccgcgggg aatacttcag 481 agagtcagag ttgtgccaga agtctcttaa tggcagatag aatgcaaata gttgtgattc 541 tctcagaatt ttttaatacc acatggcagg aggcaaattg tgcaaattgt ttaacaaaca 601 acagtgaaga attatcaaac agcacagtat atttccttaa tctatttaat cacaccctga 661 cctgctttga acataaccTT CAGGGGAATG CACATAGTCT TTTACAGACA AAAAATTATT 721 CAGAAGTATG CAAAAACTGC CGTGAAGCAT ACAAAACTCT GAGTAGTCTG TACAGTGAAA 781 TGCAAAAAAT GAATGAACTT GAGAATAAGG CTGAACCTGG AACACATTTA TGCATTGATG 841 TGGAAGATGC AATGAACATC ACTCGAAAAC TATGGAGTCG AACTTTCAAC TGTTCAGTCC 901 CTTGCAGTGA CACAGTGCCT GTAATTGCTG TTTCTGTGTT CATTCTCTTT CTACCTGTTG 961 TCTTCTACCT TAGTAGCTTT CTTCACTCAG AACAAAAGAA ACGCAAACTC ATTCTGCCCA 1021 AACGTCTCAA GTCCAGTACC AGTTTTGCAA ATATTCAGGA AAATTCAAAC TTGCCAACTT 1081 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1141 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1201 CTTGTGGAAA GGACGAAGCT TAGCACTATA GGTAGCTATC ACGCGTTAAG TCgacaatca 1261 acctctggat tacaaaattt gtgaaagatt