Construct: ORF TRCN0000476633

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015175.1_s317c1
Derived from:
ccsbBroadEn_05501
DNA Barcode:
TCCTATCACACATCCGTCCACAGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EYS (346007)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476633
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 346007 EYS eyes shut homolog NM_198283.1 100% 100%
2 human 346007 EYS eyes shut homolog NM_001142801.2 95.1% 95.3% (many diffs)
3 human 346007 EYS eyes shut homolog NM_001142800.2 18.8% 18.7% (many diffs)
4 human 346007 EYS eyes shut homolog NM_001292009.1 18.7% 18.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1851
ORF length:
1782
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gactgacaaa tcaatcgtca ttctgagcct gatggttttt cacagctctt
121tcataaatgg aaaaacatgt agacggcaat tggtggaaga atggcatcca caaccctcat
181catatgtggt aaattggaca ctaacagaaa acatctgctt ggacttctac agagattgct
241ggtttttggg tgtaaacact aaaatagata cttcaggcaa tcaagctgtt ccccagattt
301gccctttgca aattcaatta ggagatatcc ttgtaatttc ttctgaacca tctcttcaat
361ttccagaaat aaatttgatg aatgtttctg aaacatcttt cgttggctgt gtgcaaaata
421ccacaacgga agatcagtta ctttttggct gcagactaaa aggaatgcac actgttaatt
481ctaagtggct gagtgttggg acacattatt ttatcacagt tatggcaagt ggtccatcac
541cttgtccact gggacttcga ctaaatgtga cagtgaaaca gcagttctgc caggaatctc
601tgagttcaga attttgctct ggtcatggta aatgtcttag tgaagcttgg agcaagacat
661atagctgcca ttgccagcct ccattttctg gaaaatactg ccaggaactt gatgcatgtt
721cttttaaacc atgtaaaaat aatggcagtt gcattaataa aagagaaaat tgggatgagc
781aagcatatga atgtgtctgt cacccaccat ttacaggaaa gaattgctca gaaataattg
841gccagtgtca accacatgtc tgtttccatg gaaactgcag caatattact tcaaatagtt
901tcatttgtga atgtgatgag caattttcag gtccattctg tgaggtgtca gcaaaacctt
961gtgtttctct gcttttttgg aaaagaggaa tttgcccaaa tagcagttct gcttatactt
1021atgaatgccc aaaaggatct tccagccaaa atggtgaaac tgatgtcagt gagttttcat
1081tagtaccatg tcagaatggt actgactgca tcaaaatatc aaatgatgtt atgtgcatct
1141gttcaccaat atttacagat ttgctttgta agagcattca aacatcatgt gagtcatttc
1201ctttgaggaa taatgctaca tgtaagaaat gtgagaaaga ttatccttgc agctgtatat
1261caggatttac tgaaaaaaac tgtgagaaag caattgacca ctgtaaactg ctcagcatca
1321actgtctgaa tgaagaatgg tgtttcaata taattggaag attcaaatat gtatgcattc
1381cagggtgcac aaaaaatcca tgttggtttt tgaagaatgt ttacctaatt catcaacacc
1441tctgctactg tggagtcacc ttccatggta tttgccaaga taaaggTCCT GCTCAATTTG
1501AATATGTGTG GCAATTGGGA TTTGCAGGAT CTGAAGGCGA AAAGTGCCAA GGGGTTATTG
1561ATGCCTATTT CTTTCTGGCT GCAAACTGCA CTGAAGATGC AACCTATGTG AACGATCCTG
1621AAGATAATAA TTCTTCATGT TGGTTCCCAC ATGAAGGCAC AAAAGAGATT TGTGCAAATG
1681GATGCAGTTG TTTGAGTGAA GAAGACAGTC AGGAATATCG GTATCTATGT TTTCTCAGAT
1741GGGCTGGCAA CATGTATCTG GAAAATACAA CTGATGATCA AGAAAATGAG TGTCAACATG
1801AAGCTGTTTG TAAAGATGAA ATTAATAGAC CCAGGTATTT ATGTATCATT TTGCCAACTT
1861TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
1921CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
1981CTTGTGGAAA GGACGATCCT ATCACACATC CGTCCACAGC ACGCGTTAAG TCgacaatca
2041acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)