Construct: ORF TRCN0000476633
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015175.1_s317c1
- Derived from:
- ccsbBroadEn_05501
- DNA Barcode:
- TCCTATCACACATCCGTCCACAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EYS (346007)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476633
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 346007 | EYS | eyes shut homolog | NM_198283.1 | 100% | 100% | |
| 2 | human | 346007 | EYS | eyes shut homolog | NM_001142801.2 | 95.1% | 95.3% | (many diffs) |
| 3 | human | 346007 | EYS | eyes shut homolog | NM_001142800.2 | 18.8% | 18.7% | (many diffs) |
| 4 | human | 346007 | EYS | eyes shut homolog | NM_001292009.1 | 18.7% | 18.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1851
- ORF length:
- 1782
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gactgacaaa tcaatcgtca ttctgagcct gatggttttt cacagctctt 121 tcataaatgg aaaaacatgt agacggcaat tggtggaaga atggcatcca caaccctcat 181 catatgtggt aaattggaca ctaacagaaa acatctgctt ggacttctac agagattgct 241 ggtttttggg tgtaaacact aaaatagata cttcaggcaa tcaagctgtt ccccagattt 301 gccctttgca aattcaatta ggagatatcc ttgtaatttc ttctgaacca tctcttcaat 361 ttccagaaat aaatttgatg aatgtttctg aaacatcttt cgttggctgt gtgcaaaata 421 ccacaacgga agatcagtta ctttttggct gcagactaaa aggaatgcac actgttaatt 481 ctaagtggct gagtgttggg acacattatt ttatcacagt tatggcaagt ggtccatcac 541 cttgtccact gggacttcga ctaaatgtga cagtgaaaca gcagttctgc caggaatctc 601 tgagttcaga attttgctct ggtcatggta aatgtcttag tgaagcttgg agcaagacat 661 atagctgcca ttgccagcct ccattttctg gaaaatactg ccaggaactt gatgcatgtt 721 cttttaaacc atgtaaaaat aatggcagtt gcattaataa aagagaaaat tgggatgagc 781 aagcatatga atgtgtctgt cacccaccat ttacaggaaa gaattgctca gaaataattg 841 gccagtgtca accacatgtc tgtttccatg gaaactgcag caatattact tcaaatagtt 901 tcatttgtga atgtgatgag caattttcag gtccattctg tgaggtgtca gcaaaacctt 961 gtgtttctct gcttttttgg aaaagaggaa tttgcccaaa tagcagttct gcttatactt 1021 atgaatgccc aaaaggatct tccagccaaa atggtgaaac tgatgtcagt gagttttcat 1081 tagtaccatg tcagaatggt actgactgca tcaaaatatc aaatgatgtt atgtgcatct 1141 gttcaccaat atttacagat ttgctttgta agagcattca aacatcatgt gagtcatttc 1201 ctttgaggaa taatgctaca tgtaagaaat gtgagaaaga ttatccttgc agctgtatat 1261 caggatttac tgaaaaaaac tgtgagaaag caattgacca ctgtaaactg ctcagcatca 1321 actgtctgaa tgaagaatgg tgtttcaata taattggaag attcaaatat gtatgcattc 1381 cagggtgcac aaaaaatcca tgttggtttt tgaagaatgt ttacctaatt catcaacacc 1441 tctgctactg tggagtcacc ttccatggta tttgccaaga taaaggTCCT GCTCAATTTG 1501 AATATGTGTG GCAATTGGGA TTTGCAGGAT CTGAAGGCGA AAAGTGCCAA GGGGTTATTG 1561 ATGCCTATTT CTTTCTGGCT GCAAACTGCA CTGAAGATGC AACCTATGTG AACGATCCTG 1621 AAGATAATAA TTCTTCATGT TGGTTCCCAC ATGAAGGCAC AAAAGAGATT TGTGCAAATG 1681 GATGCAGTTG TTTGAGTGAA GAAGACAGTC AGGAATATCG GTATCTATGT TTTCTCAGAT 1741 GGGCTGGCAA CATGTATCTG GAAAATACAA CTGATGATCA AGAAAATGAG TGTCAACATG 1801 AAGCTGTTTG TAAAGATGAA ATTAATAGAC CCAGGTATTT ATGTATCATT TTGCCAACTT 1861 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1921 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1981 CTTGTGGAAA GGACGATCCT ATCACACATC CGTCCACAGC ACGCGTTAAG TCgacaatca 2041 acctctggat tacaaaattt gtgaaagatt