Construct: ORF TRCN0000476655
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008680.1_s317c1
- Derived from:
- ccsbBroadEn_04345
- DNA Barcode:
- TCTCTGACTATTACTTGAGCACCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MED10 (84246)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476655
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84246 | MED10 | mediator complex subunit 10 | NM_032286.3 | 100% | 100% | |
| 2 | mouse | 28077 | Med10 | mediator complex subunit 10 | NM_138596.2 | 89.6% | 99.2% | (many diffs) |
| 3 | mouse | 28077 | Med10 | mediator complex subunit 10 | XM_011244527.2 | 73.7% | 81.7% | (many diffs) |
| 4 | mouse | 28077 | Med10 | mediator complex subunit 10 | XM_006517275.3 | 55.2% | 57.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 471
- ORF length:
- 405
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggagaagttt gaccacctag aggagcacct GGAGAAGTTC GTGGAGAACA 121 TTCGGCAGCT CGGCATCATC GTCAGTGACT TCCAGCCCAG CAGCCAGGCC GGGCTCAACC 181 AAAAGCTGAA TTTTATTGTT ACTGGCTTAC AGGATATTGA CAAGTGCAGA CAGCAGCTTC 241 ATGATATTAC TGTACCGTTA GAAGTTTTTG AATATATAGA TCAAGGTCGA AATCCCCAGC 301 TCTACACCAA AGAGTGCCTG GAGAGGGCTC TAGCTAAAAA TGAGCAAGTT AAAGGCAAGA 361 TCGACACCAT GAAGAAATTT AAAAGCCTGT TGATTCAAGA ACTTTCTAAA GTATTTCCGG 421 AAGACATGGC TAAGTATCGA AGCATCCGGG GGGAGGATCA CCCGCCTTCT TACCCAACTT 481 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 541 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 601 CTTGTGGAAA GGACGATCTC TGACTATTAC TTGAGCACCC ACGCGTTAAG TCgacaatca 661 acctctggat tacaaaattt gtgaaagatt