Construct: ORF TRCN0000476684

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018434.1_s317c1
Derived from:
ccsbBroadEn_05515
DNA Barcode:
GTCACTCGCGACTCACTTAAGCTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
C2orf48 (348738)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476684
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6241 RRM2 ribonucleotide reductase re... NR_164157.1 21.9% 1_1106del;1299_1477del;1763_2178del
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
546
ORF length:
477
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggagtccagg ccctcgggga gacagcacgc cagtgaggga gatggagacc
121aatcacccac ccagtgtgcg gggatgagaa gctcgggaag gtctgaccag ccttacgtct
181tgaaagggaa tCCCCTGCTT CTGCGTGTCA GGTGTTACTC GGGGTGCGCC AGTGGAAGCG
241GGAGGCAGTT GCAGCTTTCA GTCTTCCAGG ATCTCAACCA GTTCTCTCAC TGCAGAGTCT
301GGAGAAGCCC AGCCCTCATT GTGAAGGGAG AGCCACCGTG GTGCTCACAG CAAGACACCC
361AGAGTCCCTT CCAGACAGGG ACACCCCTGG AGCGACCCTG TTTCAGAATG AAGTTATCTG
421GGTGGGAACT CACCAAGAAT GCACAGAGGG CGCTGGGCTC CAAGCTTCAG CATATCCTTT
481CACTGGACTC CACACAGGCC TGCGGAGCAG GTGGACCCAC CATTCTCAGA CCCCCCAGGG
541CCCCATTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC
601TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT
661CTTGGCTTTA TATATCTTGT GGAAAGGACG AGTCACTCGC GACTCACTTA AGCTTACGCG
721TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt

Download FASTA (ORF) (Full)