Construct: ORF TRCN0000476722
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001247.1_s317c1
- Derived from:
- ccsbBroadEn_12674
- DNA Barcode:
- ATACATTCATAGAGAATAGTAGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ASMTL-AS1 (80161)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476722
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80161 | ASMTL-AS1 | ASMTL antisense RNA 1 | NR_026710.1 | 29.9% | (many diffs) | |
| 2 | human | 80161 | ASMTL-AS1 | ASMTL antisense RNA 1 | NR_026711.1 | 20.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 606
- ORF length:
- 537
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgtcccat ggaggaaaca cactggaaat cgtgcagaag atgggaagat 121 gcaggctgga gtgcggagaa ggactgagac cctgcctggt agacggcacc gtcacagcag 181 ccgtctgcct gctaggagga gcgtctccac cagcagcagg ccggcccctg agggagacag 241 cagagagctg gagtcctggc cagccaggcg gggaTCCTTC AGCAGGACAC AGGGGACACA 301 GCAGGCAACA GGAGCTCAGA ACGCTCTCGG CGACCTTGCT GAGTAACTTG TGGACTTTGT 361 CGTCTGGCCA GTCATGCAGG ATCCGGCACA GGACGTACAG CTCAGCGCTG GGGAGGGGGT 421 CCCTGAAAAA GTCACCTGGT TTAAAGACAA AACGAGATAC GTCCGTCAGG TATGGAAGAA 481 GCAGTCCTCC CCGGACAGAT CCTGGGACGG CCACCCGCAT CCTAAATCAG GGACAGAGAA 541 GAGGGGTGTG GGCGGGAATG GGTCTTCCTG AAGTACATCC CCGTGGGGAC TTGAACAGAG 601 CTCCATTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 661 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 721 CTTGGCTTTA TATATCTTGT GGAAAGGACG AATACATTCA TAGAGAATAG TAGACACGCG 781 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt