Construct: ORF TRCN0000476759

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005414.1_s317c1
Derived from:
ccsbBroadEn_09845
DNA Barcode:
GTGGTACGACCCCACGTGATTCAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TYSND1 (219743)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476759
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 219743 TYSND1 trypsin domain containing 1 NM_001040273.3 99.7% 99.7% 333G>C;608C>T;648T>C
2 human 219743 TYSND1 trypsin domain containing 1 NM_173555.4 69.7% 67.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1260
ORF length:
1194
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgag aaggcagtgg gggtctgcca tgagggcggc cgagcaggcg ggctgcatgg
121tgagcgcctc ccgggccgga cagcccgagg cgggcccgtg gagctgcagc ggggtaatcc
181tgagccgtag cccgggcctg gtgctttgcc acgggggcat cttcgtcccc ttcctgcgag
241ctggcagcga agtcctgacc gcggccggcg ccgtcttcct gcctggcgac agttgcaggg
301acgacctgcg cctgcacgtg cagtgggccc caacggccgc gggtcccggg ggcggcgcgg
361agcggggccg cccagggctg tgcacgcccc agtgcgccag cctcgagccc ggcccacctg
421ccccgtcccg cgggcgtccc ctgcagcccc ggcttcctgc tgagctgctg ctgctgctga
481gctgcccggc cttctgggcc cacttcgcgc gcctcttcgg ggacgaggca gcggaacagt
541ggcgcttctc gagcgcggcg cgggatgacg aagtgtcgga ggacgaggag gcggatcaac
601tgagagcgct gggctggttt gcgctgctgg gcgtgcggct aggccaggag gaggtggagg
661aggagcgcgg gctagccatg gcggtgtcgc ctctcggggc cgtgcccaag ggcgcgccat
721tgctggtctg cggctcccct ttcggcgcct tctgccccga catctttctc aacacgctga
781gctgcggggt gctcagcaac gtggccggcc cactgctgct taccgacgca cgctgcctgc
841ccggcaccga gggcggcggc gtgttcaccg cgcggcccgc gggggcgctg gtggcgctgg
901tggtggcgcc gctctgttgg aaggccggcg aatgggtggg cttcacgctg ctctgcgccg
961ccgcccccct tttccgcgcc gcccgcgacg cgcttcaccg cctgccgcac agcaccgctg
1021ccctggccgc ccttctgccg ccagaggtgg gcgtcccgtg gggtctgccc ctccgagacT
1081CCGGGCCCCT GTGGGCAGCC GCGGCAGTGT TGGTGGAGTG CGGCACCGTA TGGGGCTCCG
1141GAGTGGCTGT GGCACCCCGC CTTGTAGTGA CCTGTCGGCA CGTGTCCCCT CGGGAAGCAG
1201CCAGGGTCCT GGTGCGCTCC ACCACCCCCA AGCATAATCA CCAGCAACAC CCGGGACAAT
1261ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
1321GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
1381TTTATATATC TTGTGGAAAG GACGAGTGGT ACGACCCCAC GTGATTCAGA CGCGTTAAGT
1441Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)