Construct: ORF TRCN0000476759
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005414.1_s317c1
- Derived from:
- ccsbBroadEn_09845
- DNA Barcode:
- GTGGTACGACCCCACGTGATTCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TYSND1 (219743)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476759
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 219743 | TYSND1 | trypsin domain containing 1 | NM_001040273.3 | 99.7% | 99.7% | 333G>C;608C>T;648T>C |
| 2 | human | 219743 | TYSND1 | trypsin domain containing 1 | NM_173555.4 | 69.7% | 67.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1260
- ORF length:
- 1194
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag aaggcagtgg gggtctgcca tgagggcggc cgagcaggcg ggctgcatgg 121 tgagcgcctc ccgggccgga cagcccgagg cgggcccgtg gagctgcagc ggggtaatcc 181 tgagccgtag cccgggcctg gtgctttgcc acgggggcat cttcgtcccc ttcctgcgag 241 ctggcagcga agtcctgacc gcggccggcg ccgtcttcct gcctggcgac agttgcaggg 301 acgacctgcg cctgcacgtg cagtgggccc caacggccgc gggtcccggg ggcggcgcgg 361 agcggggccg cccagggctg tgcacgcccc agtgcgccag cctcgagccc ggcccacctg 421 ccccgtcccg cgggcgtccc ctgcagcccc ggcttcctgc tgagctgctg ctgctgctga 481 gctgcccggc cttctgggcc cacttcgcgc gcctcttcgg ggacgaggca gcggaacagt 541 ggcgcttctc gagcgcggcg cgggatgacg aagtgtcgga ggacgaggag gcggatcaac 601 tgagagcgct gggctggttt gcgctgctgg gcgtgcggct aggccaggag gaggtggagg 661 aggagcgcgg gctagccatg gcggtgtcgc ctctcggggc cgtgcccaag ggcgcgccat 721 tgctggtctg cggctcccct ttcggcgcct tctgccccga catctttctc aacacgctga 781 gctgcggggt gctcagcaac gtggccggcc cactgctgct taccgacgca cgctgcctgc 841 ccggcaccga gggcggcggc gtgttcaccg cgcggcccgc gggggcgctg gtggcgctgg 901 tggtggcgcc gctctgttgg aaggccggcg aatgggtggg cttcacgctg ctctgcgccg 961 ccgcccccct tttccgcgcc gcccgcgacg cgcttcaccg cctgccgcac agcaccgctg 1021 ccctggccgc ccttctgccg ccagaggtgg gcgtcccgtg gggtctgccc ctccgagacT 1081 CCGGGCCCCT GTGGGCAGCC GCGGCAGTGT TGGTGGAGTG CGGCACCGTA TGGGGCTCCG 1141 GAGTGGCTGT GGCACCCCGC CTTGTAGTGA CCTGTCGGCA CGTGTCCCCT CGGGAAGCAG 1201 CCAGGGTCCT GGTGCGCTCC ACCACCCCCA AGCATAATCA CCAGCAACAC CCGGGACAAT 1261 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1321 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1381 TTTATATATC TTGTGGAAAG GACGAGTGGT ACGACCCCAC GTGATTCAGA CGCGTTAAGT 1441 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt