Construct: ORF TRCN0000476782
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007833.1_s317c1
- Derived from:
- ccsbBroadEn_05867
- DNA Barcode:
- GCACCTCTTAAGCAGAATAGTCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATP4B (496)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476782
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 496 | ATP4B | ATPase H+/K+ transporting s... | NM_000705.4 | 99.8% | 100% | 801G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 939
- ORF length:
- 873
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggctctgcag gagaagaaga cgtgtggcca gcgcatggag gagttccagc 121 gttactgctg gaacccggac acggggcaga tgctgggccg caccctgtcc cggtgggtgt 181 ggatcagcct gtactacgtg gccttctacg tggtgatgac tgggctcttc gccctgtgcc 241 tctatgtgct gatgcagaca gtggacccgt acacaccgga ctaccaagac cagctacggt 301 caccaggggt aaccttaagg ccggatgttt acggggagaa aggcctggaa attgtctaca 361 acgtctctga taacagaacc tgggcagacc tcacacagac tctccacgcc ttcctagcag 421 gctactctcc agcagcccag gaggacagca tcaactgcac ctccgagcag tacttcttcc 481 aggagagttt ccgcgctccc aaccacacca agttctcctg caagttcacg gcagatatgc 541 tgcagaactg cTCAGGCCTG GCGGATCCCA ACTTCGGCTT TGAAGAAGGA AAGCCATGTT 601 TTATTATTAA AATGAACAGG ATCGTCAAGT TCCTCCCCAG CAACGGCTCG GCCCCCAGAG 661 TGGACTGCGC CTTCCTGGAC CAGCCCCGCG AGCTCGGCCA GCCGCTGCAG GTCAAGTACT 721 ACCCTCCCAA CGGCACCTTC AGTCTGCACT ACTTCCCTTA TTACGGGAAG AAAGCCCAGC 781 CCCACTACAG CAACCCCCTG GTGGCAGCGA AGCTCCTCAA CATCCCCAGG AACGCTGAGG 841 TCGCCATCGT GTGCAAGGTC ATGGCAGAGC ACGTGACCTT CAACAATCCC CACGACCCGT 901 ATGAAGGGAA AGTGGAGTTC AAACTCAAGA TTGAGAAGTG CCCAACTTTC TTGTACAAAG 961 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1021 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1081 ACGAGCACCT CTTAAGCAGA ATAGTCGAAC GCGTTAAGTC gacaatcaac ctctggatta 1141 caaaatttgt gaaagatt