Construct: ORF TRCN0000476817
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018187.1_s317c1
- Derived from:
- ccsbBroadEn_05381
- DNA Barcode:
- GGTAGAAAGCCTACACAACTAGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TAFA3 (284467)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476817
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 284467 | TAFA3 | TAFA chemokine like family ... | NM_001004440.1 | 100% | 100% | |
| 2 | human | 284467 | TAFA3 | TAFA chemokine like family ... | XM_006710582.4 | 100% | 100% | |
| 3 | human | 284467 | TAFA3 | TAFA chemokine like family ... | NM_182759.2 | 78.6% | 56% | 264_265ins68;399_400ins40 |
| 4 | human | 284467 | TAFA3 | TAFA chemokine like family ... | XM_005270766.3 | 78.6% | 56% | 264_265ins68;399_400ins40 |
| 5 | mouse | 329731 | Fam19a3 | family with sequence simila... | NM_183224.3 | 68% | 46.8% | (many diffs) |
| 6 | mouse | 329731 | Fam19a3 | family with sequence simila... | XM_017319626.1 | 68% | 46.8% | (many diffs) |
| 7 | mouse | 329731 | Fam19a3 | family with sequence simila... | XM_017319625.1 | 56.1% | 39% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 576
- ORF length:
- 507
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagtgagagg gtcgagcgga actggagcac gggcggctgg ctgctggcac 121 tgtgcctggc ctggctgtgg acccacctga ccttggctgc cttgcagcct cccactgcca 181 cagtgcttgt gcagcagggc acctgcgagg tgattgcggc tcaccgctgc tgcaaccGGA 241 ACCGCATCGA GGAGCGCTCC CAGACGGTGA AATGCTCCTG TTTTTCTGGC CAGGTGGCCG 301 GCACCACGCG GGCAAAGCCC TCCTGCGTGG ACGACCTGCT CTTGGCTGCC CACTGTGCTC 361 GTAGAGACCC TAGAGCTGCA CTCCGCCTCC TGCTCCCACA GCCTCCATCG TCCTGCAGAG 421 ATGGTGGTGT CAGATGGAGC CCTGCCTGCC GGGGGAGGAG TGTAAGGTGC TCCCGGACCT 481 GTCGGGATGG AGCTGCAGCA GTGGACACAA AGTCAAAACC ACCAAGGTCA CACGATAGCT 541 CTTGGGGGTC ACGGCCTGGA CAAGAAAGGC TTGACTTGCC AACTTTCTTG TACAAAGTGG 601 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 661 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 721 AGGTAGAAAG CCTACACAAC TAGCGACGCG TTAAGTCgac aatcaacctc tggattacaa 781 aatttgtgaa agatt