Construct: ORF TRCN0000476826
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013974.1_s317c1
- Derived from:
- ccsbBroadEn_08628
- DNA Barcode:
- AATTAAATCCCCATATTCGCTGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCDHAC1 (56135)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476826
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56135 | PCDHAC1 | protocadherin alpha subfami... | NM_031882.3 | 99.8% | 99.8% | 807G>A;1269C>G;1492C>G |
| 2 | human | 56135 | PCDHAC1 | protocadherin alpha subfami... | NM_018898.3 | 84.5% | 84.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2523
- ORF length:
- 2454
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtgggctgt ggggtggcag ttttatgttt gtgggtttcc tgcggcgctg 121 cagcgggaca gctcgagtac tcagtgccgg aggagacgga gcggggcgta gccgtaggca 181 atctctccgc ggacttgagg ctgccagcgg ccgctatgtc ctcgcggaac tttcgcttcc 241 tttccagcca ccgcgagctc tacttcgggg tggatctacc cagcggcaat ttggtggtca 301 gagagccggc ggaccgcgaa cagctgtgca gggccaaagc tgcctgcgtc ttgacctacg 361 acctggtgct cgaggacccg ctggagctgc acaagattcg gattcacgtc ctggacacca 421 atgacaactc acctctcttt cctgccggcg acgtgcagct gcacatcccc gagttcctga 481 cgcccggagc ccgctttact ctcccgaatg cccaagatga cgacgaggga agcaatggga 541 tactaagcta cagcctaagc cccagtcagc actttcgcct ggacatggga tcgcgggttg 601 acggcagcga atacccggag ttggtgttgg agaaagcact ggatcgcgaa cagcgcgcca 661 cccacctgct ggtgcttaca gctcgggacg gcgggctacc tgcccgctca ggagacgcac 721 aagtcaccat cattgtggtg gacacaaatg acaacgcgcc tgtatttgag cgctccgtat 781 accgcaccaa ggttccagag actgcaccca atgggactgt gttattccga gttcaagcct 841 tggatccaga tgaagggtcc aatggggaag tccaatactc cctaagcaac agcacgcaag 901 cagagctgcg acaccgcttt cacgtgcacc ctaaaagtgg ggaggtgcaa gtagctgctt 961 cactaggtcc gcctgaaacg ctcttggagg catacattga ggcgagggac gaaggtgtct 1021 ttggtttagc tagcaccgct aaactgctgg tggaggtgac tgacgtgaac gatcatgccc 1081 ccgaactgga cttcctgact ctttcgaacc cagtacctga ggacgctgcc cctggcacag 1141 tgattgctct ctttagtgta aaggatgaag acctcgattc taatggtagg gtcatttgtg 1201 gcatgtctag tgcaggccct tttcagctga cggcttcctt tgacaactac tacagcctgc 1261 tgattgatgg gcccctggac cgggagcaga tcagtgaata ccaagtcctg atcacggcct 1321 cagatagtgg ctcaccgcca cttagcaccc gaaggacaat cactgtgtca gttgctgatg 1381 tgaatgacaa tacaccaaac tttcctcaac cccagcagga acttttcgtt gctgaaaaca 1441 atggccctgg ggcctctcta ggccgagtgt ttgcccagga ccccgacctg gggaagaatg 1501 gccttgtctc ttatgagctg ttggatgtta tctctgaagg gccatcagcc tctagcttgg 1561 tggcagtgga atcatccagt ggggccatca ctgccaaaac ttcctttgac tttgagcagc 1621 tcagggggtt tcatttccaa gtagaaggcc gggatggtgg cattcctccc agaagtgcaa 1681 cagtgactat aaacttgttt gtggtagata ggaatgacaa ttatccggtt atcttgtttc 1741 ccttgcccag aaatggttct gtcccagtgg aaattgtgcc ccgctctgcc aggactggac 1801 acttggtcac aaaagtggta gcagaggatg ctgacagtgg ttctaatgcc tggctttcct 1861 accacatctc ccgggcgtct gactctagtc tctttagaat ttcagccaat ataggtgagc 1921 tccgtactgc tcgcttagtt cttcccactg atgcagttaa gcagagggtg gtggtagtgg 1981 ttcgggacca tggagaccca ccactttcct cctctgtcac tctgggtgtg ctgttgagca 2041 actctgtccc tcagttactt ccagactttg aagatgtctg ggaaccagga gggcagcttt 2101 ctgcccagaa cttgtattta gtaattgcct tggcttgtat ttccttttta tttctggggt 2161 gcttactttt cttcgtgtgt accaagttgc accagagccc aggctgttgc gctcagagct 2221 gctgtcgctc tacagaggat cTGAGGTATG GAAGTAAGAT GGTTTCAAAT CCTTGCATGA 2281 CATCAGCCAC CATAGATGTC ACTACAGTTG AGAGACTTTC TCAGACTTAT CTCTATCGGG 2341 CCTCTCTGGG ACTTGGTTCT GATAATAACA GTTTGCTGTT GCGTGGGGAG TACAATGCTG 2401 CCGACCTGCG AAATCTTGCC ACTGGGGTAG GACTGAATTT GCCAATATCC TGTATTCAGA 2461 TTCGGAATAG GAAAGGGGAT CACGCTAATG TCAATGCCAT GGTAAGCAAA TTTTATGGAA 2521 TTTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2581 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2641 GGCTTTATAT ATCTTGTGGA AAGGACGAAA TTAAATCCCC ATATTCGCTG GAACGCGTTA 2701 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt