Construct: ORF TRCN0000476878
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000157.1_s317c1
- Derived from:
- ccsbBroadEn_06623
- DNA Barcode:
- ACTATAGTGTGCTCAATCGCCACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NDUFAB1 (4706)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476878
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4706 | NDUFAB1 | NADH:ubiquinone oxidoreduct... | NM_005003.3 | 99.7% | 99.3% | 394G>T |
| 2 | human | 4706 | NDUFAB1 | NADH:ubiquinone oxidoreduct... | XM_011545856.2 | 83.2% | 82.8% | 168_260del;487G>T |
| 3 | mouse | 70316 | Ndufab1 | NADH dehydrogenase (ubiquin... | NM_028177.3 | 86.1% | 85.2% | (many diffs) |
| 4 | mouse | 70316 | Ndufab1 | NADH dehydrogenase (ubiquin... | XM_006508200.3 | 86.1% | 85.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 534
- ORF length:
- 468
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gtctcgtgtc ctttcagcct atgtcagccg cctgcccgcg gcctttgcgc 121 cgctgccccg ggtccggatg ctggccgtgg cccggcctct cagcaccgct ctcTGCTCCG 181 CGGGGACCCA GACGAGGCTC GGGACTTTGC AGCCGGCCTT AGTGCTCGCG CAGGTTCCTG 241 GTAGAGTTAC ACAGTTGTGC CGCCAGTATA GCGACATGCC TCCTTTGACG TTAGAGGGCA 301 TCCAGGACCG TGTTCTTTAC GTATTGAAAC TCTATGACAA GATTGACCCA GAGAAGCTTT 361 CAGTAAATTC TCATTTTATG AAAGACCTGG GCTTAGACAG TTTGGACCAA GTGGAGATTA 421 TCATGGCCAT GGAAGACGAA TTTGGGTTTG AAATTCCTTA TATAGATGCT GAAAAGTTAA 481 TGTGTCCACA AGAAATTGTA GATTACATTG CAGATAAGAA GGATGTATAT GAATACCCAA 541 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 601 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 661 TATCTTGTGG AAAGGACGAA CTATAGTGTG CTCAATCGCC ACCACGCGTT AAGTCgacaa 721 tcaacctctg gattacaaaa tttgtgaaag att