Construct: ORF TRCN0000477019
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009112.1_s317c1
- Derived from:
- ccsbBroadEn_01066
- DNA Barcode:
- TTGCCATATCCCAAACTGACCGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NDUFA4 (4697)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477019
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4697 | NDUFA4 | NDUFA4 mitochondrial comple... | NM_002489.4 | 100% | 100% | |
| 2 | mouse | 17992 | Ndufa4 | NADH dehydrogenase (ubiquin... | NM_010886.2 | 85.3% | 87.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 336
- ORF length:
- 243
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGCTCCG CCAGATCATC GGTCAGGCCA 121 AGAAGCATCC GAGCTTGATC CCCCTCTTTG TATTTATTGG AACTGGAGCT ACTGGAGCAA 181 CACTGTATCT CTTGCGTCTG GCATTGTTCA ATCCAGATGT TTGTTGGGAC AGAAATAACC 241 CAGAGCCCTG GAACAAACTG GGTCCCAATG ATCAATACAA GTTCTACTCA GTGAATGTGG 301 ATTACAGCAA GCTGAAGAAG GAACGTCCAG ATTTCTTGCC AACTTTCTTG TACAAAGTGG 361 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 421 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 481 ATTGCCATAT CCCAAACTGA CCGCGACGCG TTAAGTCgac aatcaacctc tggattacaa 541 aatttgtgaa agatt