Construct: ORF TRCN0000477153
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006039.1_s317c1
- Derived from:
- ccsbBroadEn_02535
- DNA Barcode:
- GGACGACTTTAAAGACCGGTCGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CPLX2 (10814)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477153
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10814 | CPLX2 | complexin 2 | NM_001008220.2 | 100% | 100% | |
| 2 | human | 10814 | CPLX2 | complexin 2 | NM_006650.4 | 100% | 100% | |
| 3 | human | 10814 | CPLX2 | complexin 2 | XM_005265798.1 | 100% | 100% | |
| 4 | human | 10814 | CPLX2 | complexin 2 | XM_005265799.1 | 100% | 100% | |
| 5 | human | 10814 | CPLX2 | complexin 2 | XM_011534419.1 | 100% | 100% | |
| 6 | human | 10814 | CPLX2 | complexin 2 | XM_017008964.1 | 100% | 100% | |
| 7 | mouse | 12890 | Cplx2 | complexin 2 | NM_009946.3 | 92.7% | 100% | (many diffs) |
| 8 | mouse | 12890 | Cplx2 | complexin 2 | XM_017315375.1 | 92.7% | 100% | (many diffs) |
| 9 | mouse | 12890 | Cplx2 | complexin 2 | XM_017315376.1 | 92.7% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 471
- ORF length:
- 402
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggacttcgtc atgaagcagg cccttggagG GGCCACAAAG GACATGGGGA 121 AGATGCTGGG GGGAGAGGAG GAGAAGGACC CCGACGCGCA GAAAAAGGAG GAGGAGCGGC 181 AGGAGGCGCT GCGGCAGCAG GAGGAGGAGC GTAAGGCCAA GCACGCGCGC ATGGAGGCGG 241 AGCGGGAGAA GGTCCGGCAG CAGATCCGAG ATAAGTATGG GCTGAAGAAG AAGGAGGAGA 301 AGGAAGCAGA GGAGAAAGCA GCCCTGGAGC AGCCCTGCGA GGGGAGCCTG ACCCGGCCCA 361 AGAAGGCCAT CCCTGCGGGC TGCGGGGACG AGGAGGAGGA GGAAGAGGAG AGCATCCTGG 421 ACACGGTGCT CAAATACCTG CCCGGGCCGC TGCAGGACAT GTTCAAGAAG TTGCCAACTT 481 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 541 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 601 CTTGTGGAAA GGACGAGGAC GACTTTAAAG ACCGGTCGCG ACGCGTTAAG TCgacaatca 661 acctctggat tacaaaattt gtgaaagatt