Construct: ORF TRCN0000477165
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012344.1_s317c1
- Derived from:
- ccsbBroadEn_10551
- DNA Barcode:
- GGTACCACGGAAATGATCATGTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AQP4-AS1 (147429)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477165
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 112267959 | LOC112267959 | uncharacterized protein C9o... | XM_024446606.1 | 18.1% | 5% | (many diffs) |
| 2 | human | 147429 | AQP4-AS1 | AQP4 antisense RNA 1 | NR_026908.1 | 16.4% | 1_415del;809_2392del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 462
- ORF length:
- 393
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggaggatcg cagaggggca cctgccacca gcagcccagc cTCAGCACTT 121 TTCTCTTCAG CGTCTTCTTC AAGGTATTTC CAGTTGCCCG CCCTGAACTG TTTCAGAATG 181 AGGAGGACAC CTGGAGGAAT CTACACGTGC ATAGACCAAT TTGCAAAAGA ACCTGTGAGC 241 CAACATTATT ACCTCAGCTG GAAGAATTAA TAAGCAACAC GTTCACCCAA GAAGTATTTA 301 CTGAGCACCT ACTATGTGCA TTATTTTCTG GTGTTGGGGC TAGGGGGCAA ACGACTCTGA 361 CAAGTCTCTG TTCTCAAGGC TGGAGTGCAG CGGCGCGATC TCGGCTCACT GCAAGCTCCG 421 CCTCCCGGGT TCACGCCATT CTCCTGCCTC AGCCTCCCGA GTTGCCAACT TTCTTGTACA 481 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 541 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 601 AGGACGAGGT ACCACGGAAA TGATCATGTG AACGCGTTAA GTCgacaatc aacctctgga 661 ttacaaaatt tgtgaaagat t