Construct: ORF TRCN0000477347
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010936.1_s317c1
- Derived from:
- ccsbBroadEn_09343
- DNA Barcode:
- CTAATCTTTGCTGCACTCCGACGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ASB16 (92591)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477347
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 92591 | ASB16 | ankyrin repeat and SOCS box... | NM_080863.5 | 99.7% | 99.1% | (many diffs) |
| 2 | mouse | 217217 | Asb16 | ankyrin repeat and SOCS box... | NM_148953.2 | 83.1% | 85.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1428
- ORF length:
- 1359
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcaagagag accttcccct tcacctcctc catgctgcgc tctctccgcc 121 tgcagcagga gtggctggaa tgggaggacc ggcggcgggc ggctgcccag cagtgccgga 181 gccgcaggtg cccgtcaagt ccccgggccc gactcactag gcctcaccgt tcctgccgag 241 acccagctgt ccaccatgcc ctcttctccg gcaacctgca gcaggtccaa gccctgttcc 301 aagatgaaga ggccgccaac atgattgtgg agactgtgag caaccagctg gcctggtcgg 361 ctgaacaggg gttctgggtg ctgaccccca agaccaagca gacggcaccc ctcgccatcg 421 ctacagcccg aggctacaca gactgtgctc gacacctgat ccggcaggga gctgagctgg 481 atgcccgtgt cgggggtcgc gctgccttgc atgaggcctg tgcccgagcc cagtttgact 541 gtgtgcggct gctgctgacc ttcggagcca aggctaatgt gctgactgag gagggcacga 601 ctcctttgca cctctgcacg atccccgagt ccttgcagtg cgccaagttg ctgctggaag 661 caggagcgac ggtgaacctg gcagcaggcg agagccagga gacgcccctg cacgtggcgg 721 cggcgcgcgg cctggagcaa catgtggctc tgtacctgga gcatggcgcc gacgtgggcc 781 tgcgcaccag ccagggcgag actgcgctga acgcggcgtg cgctggggcc gagggcccag 841 gtagctgcag gcgacaccag gctgcggcgc gccggctcct ggaggctgga gctgatgccc 901 gggcggccgg gcgcaagcgc cacacgccgc tgcacaacgc ttgtgccagc ggctgcgggg 961 gcctggccga gctgctgctg cgttacgggg cccgcgctga ggtccccaat ggggcgggcc 1021 acacgcccat ggactgtgcg ctgcaggccg tccaggacgc ccccaactgg gagcctgaag 1081 tccttttcgc cgcactgctg gactacgggg cgcagccagt gcgcccTGAG ATGCTGAAAC 1141 ACTGCGCCAA CTTCCCTCGG GCCCTGGAAG TCCTGCTTAA TGCCTATCCT TGTGTCCCAT 1201 CCTGTGAGAC CTGGGTGGAG GCGGTGCTCC CAGAGCTGTG GAAGGAGCAC GAAGCCTTCT 1261 ACAGCTCGGC CCTGTGCATG GTGAACCAGC CAAGGCAGCT GCAGCACCTG GCCCGACTAG 1321 CTGTGCGCGC TCGGTTGGGA AGCCGCTGCC GGCAGGGTGC CACCCGGCTG CCACTGCCCC 1381 CGCTCCTCAG GGACTACCTG CTGCTGCGTG TGGAGGGGTG CATCCAGTTG CCAACTTTCT 1441 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1501 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1561 GTGGAAAGGA CGACTAATCT TTGCTGCACT CCGACGTACG CGTTAAGTCg acaatcaacc 1621 tctggattac aaaatttgtg aaagatt