Construct: ORF TRCN0000477390
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003730.1_s317c1
- Derived from:
- ccsbBroadEn_04098
- DNA Barcode:
- TGGGGATCAGCGCGACCATTTAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PANK3 (79646)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477390
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79646 | PANK3 | pantothenate kinase 3 | NM_024594.4 | 100% | 100% | |
| 2 | mouse | 211347 | Pank3 | pantothenate kinase 3 | NM_145962.2 | 93.3% | 99.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1176
- ORF length:
- 1110
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gatcaaagat gccaagaaac cctctttccc atggtttggc atggacattg 121 ggggaactct agtaaagctc tcgtactttg aacctattga tatcacagca gaggaagagc 181 aagaagaagt tgagagttta aaaagtattc ggaaatattt gacttctaac gtggcatatg 241 gatccaccgg cattcgggat gtacaccttg aactgaaaga tttaacactt tttggccgaa 301 gagggaactt gcactttatc aggtttccaa cccaggacct gcctactttt atccaaatgg 361 gaagagataa aaacttctca acattgcaga cggtgctatg tgctacagga ggtggtgctt 421 acaagtttga aaaagatttt cgcacaattg gaaacctcca cctgcacaaa ctggatgaac 481 ttgactgcct tgtaaagggc ttgctgtata tagactctgt cagtttcaat ggacaagccg 541 agtgctatta ttttgctaat gcctcagaac ctgagcgatg ccaaaagatg ccttttaacc 601 tggatgatcc ctatccactg cttgtagtga acattggctc aggagtcagt attttagcag 661 tccattccaa agacaactat aaacgagtga ctgggacaag ccttggaggg ggtaccTTTC 721 TGGGTTTATG CAGTTTATTG ACTGGCTGTG AAAGTTTTGA AGAGGCTCTT GAAATGGCAT 781 CCAAAGGTGA TAGCACACAA GCTGACAAGC TGGTCCGTGA TATTTATGGA GGAGATTATG 841 AAAGATTTGG TTTGCCAGGT TGGGCTGTAG CATCTAGTTT TGGGAATATG ATTTATAAGG 901 AGAAGCGAGA ATCTGTTAGT AAAGAAGATC TGGCAAGAGC TACTTTAGTT ACTATCACCA 961 ATAACATTGG TTCTGTGGCA CGAATGTGTG CTGTTAATGA GAAAATAAAC AGAGTTGTCT 1021 TTGTTGGAAA CTTTTTACGT GTCAATACCC TCTCAATGAA ACTTTTGGCA TATGCACTGG 1081 ATTACTGGTC AAAAGGTCAA CTAAAAGCAT TGTTTCTAGA ACATGAGGGT TACTTTGGAG 1141 CAGTTGGTGC ACTTCTTGGG CTGCCAAATT TCAGCTACCC AACTTTCTTG TACAAAGTGG 1201 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1261 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1321 ATGGGGATCA GCGCGACCAT TTAGTACGCG TTAAGTCgac aatcaacctc tggattacaa 1381 aatttgtgaa agatt