Construct: ORF TRCN0000477391
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005181.1_s317c1
- Derived from:
- ccsbBroadEn_10132
- DNA Barcode:
- CAAACCAAATCTGAGACGTGACGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OR2A7 (401427)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477391
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 401427 | OR2A7 | olfactory receptor family 2... | NM_001005328.1 | 99.7% | 99.6% | 171C>A;526T>N |
| 2 | human | 107987545 | LOC107987545 | olfactory receptor 2A7 | XM_024452660.1 | 99.7% | 99.6% | 171C>A;526T>N |
| 3 | human | 79541 | OR2A4 | olfactory receptor family 2... | NM_030908.2 | 99.3% | 98.7% | (many diffs) |
| 4 | human | 392138 | OR2A25 | olfactory receptor family 2... | NM_001004488.1 | 82.8% | 79.3% | (many diffs) |
| 5 | human | 728377 | ARHGEF34P | Rho guanine nucleotide exch... | NR_033942.1 | 14% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 999
- ORF length:
- 930
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggggacaat ataacatcca tcacagagtt cctcctactg ggatttcccg 121 ttggcccaag gattcagatg ctcctctttg ggctcttctc cctgttctac gtcttcaccc 181 tgctggggaa cgggaccata ctggggctca tctcactgga ctccagactg cacgccccaa 241 tgtacttctt cctctcacac ctggcggtcg tcgacatcgc ctacgcctgc aacacggtgc 301 cccggatgct ggtgaacctc ctgcatccag ccaagcccat ctcctttgcg ggccgcatga 361 tgcagacctt tctgttttcc acttttgctg tcacagaatg tctcctcctg gtggtgatgt 421 cctatgatct gtacgtggcc atctgccacc ccctccgata tttggccatc atgacctgga 481 gagtctgcat caccctcgcg gtgacttcct ggaccactgg agtcctttta tccttgattc 541 atcttgtgtt acttctacct ttacccttct gtaggcccca gaaaatttat cacntttttt 601 gtgaaatctt ggctgttctc aaacttgcct gtgcagatac ccacatcaat gagaacatgg 661 TCTTGGCCGG AGCAATTTCT GGGCTGGTGG GACCCTTGTC CACAATTGTA GTTTCATATA 721 TGTGCATCCT CTGTGCTATC CTTCAGATCC AATCAAGGGA AGTTCAGAGG AAAGCCTTCT 781 GCACCTGCTT CTCCCACCTC TGTGTGATTG GACTCTTTTA TGGCACAGCC ATTATCATGT 841 ATGTTGGACC CAGATATGGG AACCCCAAGG AGCAGAAGAA ATATCTCCTG CTGTTTCACA 901 GCCTCTTTAA TCCCATGCTC AATCCCCTTA TCTGTAGTCT TAGGAACTCA GAAGTGAAGA 961 ATACTTTGAA GAGAGTGCTG GGAGTAGAAA GGGCTTTATT GCCAACTTTC TTGTACAAAG 1021 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1081 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1141 ACGACAAACC AAATCTGAGA CGTGACGTAC GCGTTAAGTC gacaatcaac ctctggatta 1201 caaaatttgt gaaagatt