Construct: ORF TRCN0000477490
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003263.1_s317c1
- Derived from:
- ccsbBroadEn_11614
- DNA Barcode:
- AGCGCACTGATAAGTAGACTCTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GALNT5 (11227)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477490
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11227 | GALNT5 | polypeptide N-acetylgalacto... | XM_017003238.2 | 96.6% | 96.6% | 230C>T;1455_1503delinsG |
| 2 | human | 11227 | GALNT5 | polypeptide N-acetylgalacto... | NM_014568.3 | 51.5% | 51.4% | 230C>T;1455_2820delinsG |
| 3 | human | 11227 | GALNT5 | polypeptide N-acetylgalacto... | XM_017003237.2 | 50.6% | 50.6% | 230C>T;1455_2868delinsG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1524
- ORF length:
- 1455
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaacaggatc cgaaagtttt tccgaggaag tgggcgagtc ttggcattta 121 tctttgtagc ttctgtcatc tggctcctct ttgacatggc agctctccgc ctctcattca 181 gtgagatcaa cactcgggtc atcaaggaag acattgtgag gagggagcgg ataggattca 241 gagttcagcc agaccaagga aaaatttttt acagcagcat aaaagagatg aaacctctcc 301 taaggggaca tgggaaaggg gcatggggca aagagaatgt tagaaaaact gaggagagtg 361 tgctcaaggt tgaggtggac ttggaccaaa cccagaggga aagaaaaatg cagaatgccc 421 tgggaagggg caaggttgtg ccgttgtggc atcctgcaca tctgcagacc ctccctgtga 481 ctcctaacaa gcagaagaca gacgggagag gcaccaaacc tgaagcctcc tctcaccagg 541 ggacaccaaa gcaaacgaca gctcaggggg ctccaaagac ctcattcata gcagcaaaag 601 gaactcaggt agtcaaaata tcagtacaca tgggacgtgt cagtttaaaa caggagcccc 661 ggaagagtca tagtcccagc agtgacacat caaaactagc agctgaaagg gacttgaatg 721 tgaccatcag tcttagtact gatagaccaa agcagcgatc acaggcagta gcaaacgaga 781 gggcacaccc tgccagcaca gcagtgccga agtctgggga agccatggcc ttaaacaaaa 841 ctaagactca gagcaaagaa gtcaatgcaa ataaacacaa agccaatacg agtcttcctt 901 ttcctaagtt cactgtcaat tcaaatcgct taaggaagca atctattaat gagacacctt 961 tgggaagttt gtcaaaggat gatggagcta gaggggctca tgggaagaaa ctcaatttct 1021 ctgaaagcca tcttgtgatt ataaccaaag aggaagagca aaaggcagac cccaaagagg 1081 tctctaattc taaaaccaaa acaatatttc cTAAAGTATT GGGTAAAAGC CAAAGTAAAC 1141 ACATTTCCAG GAATAGAAGT GAGATGTCTT CCTCTTCACT TGCTCCACAT AGAGTGCCAC 1201 TGTCCCAAAC TAACCATGCT TTAACTGGAG GGCTAGAGCC AGCAAAAATC AACATAACTG 1261 CCAAAGCCCC CTCTACAGAA TACAACCAGA GTCATATAAA AGCCCTTTTA CCTGAAGACA 1321 GTGGAACGCA CCAGGTGTTA AGAATTGATG TGACACTTTC TCCAAGGGAC CCCAAAGCTC 1381 CAGGGCAGTT TGGGCGTCCT GTAGTTGTCC CCCATGGAAA GGAGAAGGAG GCAGAAAGAA 1441 GATGGAAAGA AGGAAACTTC AATGTCTACC TTAGCGATTT GATCCCAGTG GATAGAGCCA 1501 TTGAAGACAC CAGACCTGCT GGGTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1561 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1621 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA GCGCACTGAT 1681 AAGTAGACTC TTCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1741 att