Construct: ORF TRCN0000477496
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009135.1_s317c1
- Derived from:
- ccsbBroadEn_01963
- DNA Barcode:
- ATACCCAAGTAAATGAACCCGGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OR6A2 (8590)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477496
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8590 | OR6A2 | olfactory receptor family 6... | NM_003696.2 | 100% | 100% | |
| 2 | mouse | 18317 | Olfr2 | olfactory receptor 2 | NM_010983.2 | 87.2% | 89.9% | (many diffs) |
| 3 | mouse | 18317 | Olfr2 | olfactory receptor 2 | XM_006507431.1 | 87.2% | 89.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1050
- ORF length:
- 981
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagtggcgg aaccatagtg ggagagtgag tgagtttgtg ttgctgggct 121 tccctgctcc tgcgccacta caggtactat tgtttgccct tttgctgctg gcctatgtgt 181 tggtgctgac tgagaacaca ctcatcatta tggcaattag gaaccattct accctccaca 241 aacccatgta cttttttcta gctaatatgt cctttctgga gatctggtat gtcactgtca 301 ctattcccaa gatgcttgct ggctttgttg gatccaaaca ggatcatgga cagctaatct 361 cctttgaggg atgcatgaca cagctctact ttttccttgg cttgggctgc actgagtgtg 421 tccttctcgc tgttatggcc tatgatcgct atatggccat ctgctatcct ctccactacc 481 cagtcattgt cagtggccgg ctgtgtgtgc agatggctgc tggctcttgg gctggaggtt 541 ttggcatctc catggtcaaa gtttttctta tttctggcct ctcttactgt ggccccaaca 601 tcatcaacca ctttttctgt gatgtctctc cattgctcaa cctctcatgc acTGATATGT 661 CCACAGCAGA GCTTACAGAT TTCATCCTGG CCATTTTTAT TCTTCTAGGG CCACTCTCTG 721 TCACTGGGGC CTCCTATGTG GCCATTACTG GTGCTGTGAT GCACATTCCT TCGGCTGCTG 781 GACGCTATAA GGCCTTTTCC ACCTGTGCCT CTCATCTCAC TGTTGTGATA ATCTTCTATG 841 CAGCCAGTAT CTTCATCTAT GCTCGGCCAA AGGCACTCTC AGCTTTTGAC ACCAACAAGT 901 TGGTCTCTGT ACTGTATGCT GTCATTGTAC CATTGCTCAA TCCCATCATT TACTGCCTGC 961 GCAATCAAGA GGTCAAGAGA GCCCTATGCT GTACTCTGCA CCTGTACCAG CACCAGGATC 1021 CTGACCCCAA GAAAGCTAGC AGAAATGTAT TGCCAACTTT CTTGTACAAA GTGGTTGATA 1081 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1141 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAATACC 1201 CAAGTAAATG AACCCGGTTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1261 tgaaagatt