Construct: ORF TRCN0000477503
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015329.1_s317c1
- Derived from:
- ccsbBroadEn_10631
- DNA Barcode:
- ATACTGCCCTTCCAAGCCGGACGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATP6V0E2-AS1 (401431)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477503
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 401431 | ATP6V0E2-AS1 | ATP6V0E2 antisense RNA 1 | NR_027040.1 | 13.9% | 1_70del;259T>G;503_3084del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 501
- ORF length:
- 432
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggttatagg gtccgcgTCA GCCCCGCGCC CGCACAGCCA ACGTCACGCC 121 GCATTCTTAT TTCCAAAGAC CCCTGGAGCT TGGGGTCCCA CCCCAGCGGT CTTTCGGGGC 181 GTCCCCTGCC CTTCACGCGC CGCGCCGGCT CGGCCCGAAG CGCCCTGCGG ACCCGCAGCC 241 TGGGCCGCCC CGAACCGGAC GGCCCCGCGG GGGTCGGACG CGGGAGGAAC GGCCTCGCTT 301 TCAGAGACGC AGCGGGAGGG CGGTCCCCGC TGTCCCTCTG GCCGGGTCCT CAGCACGCGT 361 TCTCCTCACT CCCAGGACTA GCGAGCGGCG GGCGCGGACC GGGGCCTGGG GTCGGAGGCG 421 GCGCAGCCAG AAAGGCCCCA GCGGCGCTGA GACGTTTGGG GGAAGGCGGG GGCTTCGCGT 481 TTCGATGGCG GGAAGCGGAA TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 541 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 601 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAATAC TGCCCTTCCA 661 AGCCGGACGC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt