Construct: ORF TRCN0000477507
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016755.1_s317c1
- Derived from:
- ccsbBroadEn_06018
- DNA Barcode:
- TGCTCAAGATTAGCGTTCGAAATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COMT (1312)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477507
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1312 | COMT | catechol-O-methyltransferase | NM_000754.4 | 99.8% | 100% | 408C>G |
| 2 | human | 1312 | COMT | catechol-O-methyltransferase | NM_001135161.2 | 99.8% | 100% | 408C>G |
| 3 | human | 1312 | COMT | catechol-O-methyltransferase | NM_001135162.2 | 99.8% | 100% | 408C>G |
| 4 | human | 1312 | COMT | catechol-O-methyltransferase | NM_001362828.2 | 99.8% | 100% | 408C>G |
| 5 | human | 1312 | COMT | catechol-O-methyltransferase | NM_007310.3 | 81.4% | 81.5% | 0_1ins150;258C>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 879
- ORF length:
- 813
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc ggaggccccg cctctgctgt tggcagctgt gttgctgggc ctggtgctgc 121 tggtggtgct gctgctgctt ctgaggcact ggggctgggg cctgtgcctt atcggctgga 181 acgagttcat cctgcagccc atccacaacc tgctcatggg tgacaccaag gagcagcgca 241 tcctgaacca cgtgctgcag catgcggagc ccgggaacgc acagagcgtg ctggaggcca 301 ttgacaccta ctgcgagcag aaggagtggg ccatgaacgt gggcgacaag aaaggcaaga 361 tcgtggacgc cgtgattcag gagcaccagc cctccgtgct gctggagctg ggggcctact 421 gtggctacTC AGCTGTGCGC ATGGCCCGCC TGCTGTCACC AGGGGCGAGG CTGATCACCA 481 TCGAGATCAA CCCCGACTGT GCCGCCATCA CCCAGCGGAT GGTGGATTTC GCTGGCGTGA 541 AGGACAAGGT CACCCTTGTG GTTGGAGCGT CCCAGGACAT CATCCCCCAG CTGAAGAAGA 601 AGTATGATGT GGACACACTG GACATGGTCT TCCTCGACCA CTGGAAGGAC CGGTACCTGC 661 CGGACACGCT TCTCTTGGAG GAATGTGGCC TGCTGCGGAA GGGGACAGTG CTACTGGCTG 721 ACAACGTGAT CTGCCCAGGT GCGCCAGACT TCCTAGCACA CGTGCGCGGG AGCAGCTGCT 781 TTGAGTGCAC ACACTACCAA TCGTTCCTGG AATACAGGGA GGTGGTGGAC GGCCTGGAGA 841 AGGCCATCTA CAAGGGCCCA GGCAGCGAAG CAGGGCCCTG CCCAACTTTC TTGTACAAAG 901 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 961 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1021 ACGATGCTCA AGATTAGCGT TCGAAATTAC GCGTTAAGTC gacaatcaac ctctggatta 1081 caaaatttgt gaaagatt