Construct: ORF TRCN0000477553
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017470.1_s317c1
- Derived from:
- ccsbBroadEn_09991
- DNA Barcode:
- GCCTGCTACAGTAGCATCGTCAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KRT6C (286887)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477553
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 286887 | KRT6C | keratin 6C | NM_173086.5 | 99.9% | 99.8% | 545G>A |
| 2 | human | 3854 | KRT6B | keratin 6B | NM_005555.4 | 98.4% | 98.5% | (many diffs) |
| 3 | human | 3853 | KRT6A | keratin 6A | NM_005554.4 | 97.8% | 98.2% | (many diffs) |
| 4 | mouse | 16687 | Krt6a | keratin 6A | NM_008476.3 | 85.5% | 85.6% | (many diffs) |
| 5 | mouse | 16688 | Krt6b | keratin 6B | NM_010669.2 | 85% | 84.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1761
- ORF length:
- 1692
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccagcaca tccaccacca tcaggagcca cagcagcagc cgccggggtt 121 tcagtgccaa ctcagccagg ctccctgggg tcagccgctc tggcttcagc agcatctccg 181 tgtcccgctc caggggcagt ggtggcctgg gtggtgcatg tggaggagct ggctttggca 241 gccgcagtct gtatggcctg gggggctcca agaggatctc cattggaggg ggcagctgtg 301 ccatcagtgg cggctatggc agcagagccg gaggcagcta tggctttggt ggcgccggga 361 gtggatttgg tttcggtggt ggagccggca ttggctttgg tctgggtggt ggagccggcc 421 ttgctggtgg ctttgggggc cctggcttcc ctgtgtgccc ccctggaggc atccaagagg 481 tcaccgtcaa ccagagtctc ctgactcccc tcaacctgca aattgacccc gccatccagc 541 gggtgcgggc cgaggagcgt gagcagatca agaccctcaa caacaagttt gcctccttca 601 tcgacaaggt gcagttccta gagcagcaga acaaggttct ggacaccaag tggaccctgc 661 tgcaggagca gggcaccaag actgtgaggc agaacctgga gccgttgttc gagcagtaca 721 tcaacaacct caggaggcag ctggacagca tcgtcgggga acggggccgc ctggactcgg 781 agctgagaaa catgcaggac ctggtggagg acctcaagaa caaatatgag gatgaaatca 841 acaagcgcac agcagcagag aatgaatttg tgactctgaa gaaggatgtg gatgctgcct 901 acatgaacaa ggttgaactg caagccaagg cagacactct cacagatgag atcaacttcc 961 tgagagcctt gtatgatgca gagctgtccc agatgcagac ccacatctca gacacatccg 1021 tggtgctatc catggacaac aaccgcaacc tggacctgga cagcatcatc gctgaggtca 1081 aggcccaata cgaggagatt gctcagagga gccgggctga ggctgagtcc tggtaccaga 1141 ccaagtacga ggagctgcag gtcacagcag gcagacatgg ggacgacctg cgcaacacca 1201 agcaggagat tgctgagatc aaccgcatga tccagaggct gagatctgag atcgaccatg 1261 tcaagaagca gtgtgccagc ctgcaggctg ccattgctga tgctgagcag cgtggggaga 1321 tggcactcaa ggatgctaag aacaagctgg aagggctgga ggatgccctg cagaaggcca 1381 agcaggacct ggcccggctg ctgaaggagt accaggagct gatgaatgtc aagctggccc 1441 tggatgtgga gatcgccacc taccgcaagc tgctggaggg cgaggagtgc aggctgaatg 1501 gcgaaggcgt tggacaagtc aacgtctctg tagtacagtc caccatctcc agtggctatg 1561 gcggtgccag cggtgtcggc agtggcttag gcctgggtgg aggaagcagc tactcctatg 1621 gcagtggtct tggcattgga ggtggcttca gttccagcag tggcagagcc attgggggtg 1681 gcctcagctc tgttggaggc ggcagttcca ccatcaagta caccaccacc tcctccTCCA 1741 GCAGGAAGAG CTACAAGCAC TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1801 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1861 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGCCT GCTACAGTAG 1921 CATCGTCAGT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt