Construct: ORF TRCN0000477595
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002476.1_s317c1
- Derived from:
- ccsbBroadEn_08588
- DNA Barcode:
- TTATGAAAGACGTGTCCCACCTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DDX28 (55794)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477595
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55794 | DDX28 | DEAD-box helicase 28 | NM_018380.4 | 99.9% | 99.8% | 10A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1686
- ORF length:
- 1620
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tctagcgcgg ccggtgcggc tcttttccct cgtgactcgg ttgctcctgg 121 cgccgcgacg gggcctcacg gtccgcagtc ccgacgaacc cctgccggtg gtgcgcatcc 181 cagtggctct acagcggcag ttggaacagc ggcagagcag gcggcggaac ctcccgaggc 241 cggtgctggt tcgacccgga ccgctgctgg tttcggcgcg gcggccggag ttgaaccagc 301 cggcgcgcct cacactgggc cgttgggagc gcgcgccgct agcctctcaa ggctggaaga 361 gtcgacgcgc gcgtcgggac cacttctcca tcgagcgcgc gcaacaggag gcgccagcgg 421 tgcgaaagct ctcgtctaag ggcagctttg ctgacctggg cctggagccc cgtgtgctgc 481 acgcactaca ggaggctgcg cctgaagtcg ttcagcccac aaccgtgcag tctagcacca 541 tcccctcact acttcgcggc cgccatgtcg tttgcgccgc agaaaccggc agtggcaaga 601 ctctcagcta cctcctgccg ctgcttcaac ggctcttggg ccagccaagc ctggactccc 661 ttcctatccc cgcgccccga ggcctggtcc ttgttccttc ccgagaattg gcccaacagg 721 tgcgggctgt ggcccaaccc ttgggccgct ccttgggcct gctggtgcgg gacctggagg 781 gaggccacgg catgcgtagg atcaggctgc agctgtccag acagccttca gcagatgtgc 841 ttgtggccac tccaggggct ctgtggaagg ccctgaaaag tcgactgatc agtctggagc 901 aactctcctt cttggtgttg gatgaggcag acacactgct ggatgaaagc ttcctggaac 961 tggtggacta catcttagag aagagccaca tagcagaagg cccagctgac ttggaagacc 1021 ccttcaatcc caaagctcag ttagtgctgg taggagccac atttcccgaa ggtgtaggcc 1081 agttgctgaa taaagtcgcc agcccagatg ctgtcaccac catcaccagc tccaagctcc 1141 actgtatcat gcctcatgtg aaacagacat ttctgagact gaagggagca gataaggtgg 1201 ccgagctggt gcacatcctc aagcatcgtg acagagcaga aaggactggt ccctcaggaa 1261 ctgttctggt gttctgtaat agctccagca ctgtgaactg gctgggatat attctggatg 1321 accacaaaat ccaacaccta aggttgcagg ggcaaatgcc agccttgatg agggtaggaa 1381 tcttccagtc cTTCCAGAAG AGCTCCCGAG ACATACTTCT CTGCACAGAC ATAGCCTCTC 1441 GGGGCCTGGA CAGCACTGGT GTGGAGCTGG TTGTCAATTA TGATTTCCCC CCAACGCTGC 1501 AAGATTACAT CCACAGAGCA GGGAGAGTGG GCCGTGTGGG GAGCGAGGTG CCAGGCACCG 1561 TCATCAGTTT TGTGACCCAT CCCTGGGATG TGAGCCTGGT TCAGAAGATT GAGCTGGCGG 1621 CTCGCCGAAG GAGAAGTCTT CCAGGACTAG CATCCTCGGT GAAAGAGCCT TTGCCCCAAG 1681 CAACCTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1741 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1801 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATTATGAAAG ACGTGTCCCA CCTATACGCG 1861 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt