Construct: ORF TRCN0000477596
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012791.1_s317c1
- Derived from:
- ccsbBroadEn_04572
- DNA Barcode:
- AGCGTTGACAGAAGAATTTGCACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RCSD1 (92241)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477596
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 92241 | RCSD1 | RCSD domain containing 1 | NM_052862.4 | 100% | 100% | |
| 2 | human | 92241 | RCSD1 | RCSD domain containing 1 | NM_001322923.1 | 92.7% | 92.7% | 103_104ins90 |
| 3 | human | 92241 | RCSD1 | RCSD domain containing 1 | NM_001322924.2 | 87% | 87% | 106_107ins162 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1317
- ORF length:
- 1248
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaggaaaga ccggcagaga ccaatgccaa tgtggacaac tcggcgtccc 121 cctcggtggc ccagctggcc gggcggttta gggagcaggc ggctgcagcc aaggagacac 181 cagccagtaa accaacccga aggaaaccgc cctgttccct ccccctgttc ccccccaagg 241 tagacctggg ccagaatggt gaggagaaat caccacccaa tgcgagccac cctcctaaat 301 tcaaggtcaa gagctcgcct ctgattgaga agcttcaggc caatttaacc tttgacccag 361 ctgctctact gcctggggcc tcacccaaga gtcctggact caaggctatg gtgtcgccat 421 ttcacagccc accttctacc cccagcagcc ctggtgtgcg atctaggccc agcgaggcag 481 aggaggtgcc tgtcagcttc gaccagcccc ctgaaggcag tcatctgccc tgttacaaca 541 aggtgcggac gaggggctca ataaaaaggc gccctccctc caggcgattc cgaaggtcac 601 agtcagactg tggagaactt ggagatttca gggcggtgga gtcatctcag cagaacggtg 661 ctaaggaaga ggatggggat gaagtgttgc catccaagag caaggcccca ggatcccctt 721 tgtccagtga gggagcagcg ggagagggag tgagaaccct gggacctgct gaaaagcctc 781 ctctgaggag gtcacccagc aggacagaga agcaggagga ggacagggcc acagaggaag 841 ccaagaacgg tgaaaaggcc aggcggagtt cagaggaggt ggacggccag cacccggccc 901 aagaggaggt cccggaatcg ccccagacct ctggcccaga ggcagaaaat aggtgtggga 961 gccccaggga ggaaaagcca gctggagagg aagcagagat ggaaaaGGCT ACAGAGGTGA 1021 AGGGGGAGAG GGTGCAAAAT GAAGAGGTGG GACCTGAACA TGACAGCCAA GAAACAAAGA 1081 AGCTGGAGGA GGGAGCTGCA GTGAAGGAGA CCCCCCACAG TCCCCCTGGA GGAGTGAAGG 1141 GCGGAGATGT CCCCAAGCAG GAAAAAGGCA AGGAAAAACA ACAGGAGGGG GCAGTGCTCG 1201 AGCCAGGCTG CAGCCCCCAG ACCGGCCCTG CCCAGCTGGA GACCAGCAGT GAGGTCCAGA 1261 GCGAGCCAGC AGTCCCCAAG CCGGAGGATG ACACTCCTGT CCAGGACACT AAAATGTTGC 1321 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1381 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1441 ATATATCTTG TGGAAAGGAC GAAGCGTTGA CAGAAGAATT TGCACGACGC GTTAAGTCga 1501 caatcaacct ctggattaca aaatttgtga aagatt