Construct: ORF TRCN0000477602

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012948.1_s317c1
Derived from:
ccsbBroadEn_13685
DNA Barcode:
ACCGAGCTCGCTTGTGCGCTGGGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
FER1L6-AS1 (439941)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477602
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 439941 FER1L6-AS1 FER1L6 antisense RNA 1 NR_040044.1 17.8% 1_177del;592_2315del
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
483
ORF length:
414
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggacatccTC CCTTACCTTC ACATGTCACA TGGCAAGTGC CCTCTCCTGG
121TGAGGGGCAA GGGTGAGATG GAGGGAGAAG CCCTGTTGAG CTGCTTGGCC ATGAACAGTT
181TGGGGGAACA GGAAGCTTGC CTTGACCTCG GGTCCAAGAC TCCCTCCCTA GAGATCTCAT
241CAAATAACCA GGAAAGACCA ACCAACAGAG AAGAGACTGG AATCATCTGC CCAGAAAGAC
301TTTTCATCTA TTCTTCTAAG GACAGCTCCA AGAGATTACC TGGGGGACTT TGTATAAAAA
361ATAAGACAAC CTGTGTTCCT GTGCAGCTTC ATCCCTCATC TTTCCCTAAG TGTCAAGAAG
421CAATAGTTAG TCCACAAGCA CGAGTGAAAT TACTGAACAA AATTAAGATG GATACAGCAC
481TCTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
541TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
601GGCTTTATAT ATCTTGTGGA AAGGACGAAC CGAGCTCGCT TGTGCGCTGG GTACGCGTTA
661AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)