Construct: ORF TRCN0000477602
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012948.1_s317c1
- Derived from:
- ccsbBroadEn_13685
- DNA Barcode:
- ACCGAGCTCGCTTGTGCGCTGGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FER1L6-AS1 (439941)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477602
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 439941 | FER1L6-AS1 | FER1L6 antisense RNA 1 | NR_040044.1 | 17.8% | 1_177del;592_2315del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 483
- ORF length:
- 414
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggacatccTC CCTTACCTTC ACATGTCACA TGGCAAGTGC CCTCTCCTGG 121 TGAGGGGCAA GGGTGAGATG GAGGGAGAAG CCCTGTTGAG CTGCTTGGCC ATGAACAGTT 181 TGGGGGAACA GGAAGCTTGC CTTGACCTCG GGTCCAAGAC TCCCTCCCTA GAGATCTCAT 241 CAAATAACCA GGAAAGACCA ACCAACAGAG AAGAGACTGG AATCATCTGC CCAGAAAGAC 301 TTTTCATCTA TTCTTCTAAG GACAGCTCCA AGAGATTACC TGGGGGACTT TGTATAAAAA 361 ATAAGACAAC CTGTGTTCCT GTGCAGCTTC ATCCCTCATC TTTCCCTAAG TGTCAAGAAG 421 CAATAGTTAG TCCACAAGCA CGAGTGAAAT TACTGAACAA AATTAAGATG GATACAGCAC 481 TCTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 541 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 601 GGCTTTATAT ATCTTGTGGA AAGGACGAAC CGAGCTCGCT TGTGCGCTGG GTACGCGTTA 661 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt