Construct: ORF TRCN0000477644
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013863.1_s317c1
- Derived from:
- ccsbBroadEn_09971
- DNA Barcode:
- CTATACGTGATCCTTTTTCTAACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C20orf203 (284805)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477644
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 284805 | C20orf203 | chromosome 20 open reading ... | NM_182584.4 | 99.8% | 100% | 192G>A |
| 2 | human | 284805 | C20orf203 | chromosome 20 open reading ... | XM_024451878.1 | 99.8% | 100% | 192G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 651
- ORF length:
- 582
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtttcctagg cctgtcttga actcccgggc tcaagcgatt ctcctgcctc 121 agcctcccaa catgctggat cacaggcagt ggccacctcg cctggccagt tttcccttta 181 caaaaactgg aatgctgagc cgggccacat cagtcttggc tggactcact gcccacctct 241 gggaccttgg cggtggggca ggaaggagga cctcaaaggc tcagcgagtc cacccccagc 301 ccagccacca gcgccagcct cctcctccgc aacacccagg cccttatcag gaaaggattt 361 gggttggtgg ggaggggtgg ggggaagttg gaggcctcag gctcagcaag gtgggtcgga 421 gagataggga agtggGGAGG GGGCTGCGGG CCCCTGCTGG GCGGGGGAGG GCAATGGGAG 481 GGATGCCCAG AATGGGCACG GTGGGGGACT TTGGCCAAGC TCTGTCCTCG CTGGCCTGGA 541 CCTCAACATG TTTCCAGGAC TTCTGCCTCC CATCCTTGCC AGGCAAGTTG CCTGCACCTT 601 TAATTAGCAA GCAGCAGTTT CTCTCCAATT CATCACGGTC GCTGTTTAAT TTGCCAACTT 661 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 721 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 781 CTTGTGGAAA GGACGACTAT ACGTGATCCT TTTTCTAACG ACGCGTTAAG TCgacaatca 841 acctctggat tacaaaattt gtgaaagatt