Construct: ORF TRCN0000477648
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012877.1_s317c1
- Derived from:
- ccsbBroadEn_11121
- DNA Barcode:
- AACGAATCGGCCCAGTCCGCGACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCL4 (6351)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477648
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6351 | CCL4 | C-C motif chemokine ligand 4 | NM_002984.4 | 100% | 100% | |
| 2 | human | 388372 | CCL4L1 | C-C motif chemokine ligand ... | NM_207007.3 | 97.4% | 96.7% | (many diffs) |
| 3 | human | 9560 | CCL4L2 | C-C motif chemokine ligand ... | NM_001291468.2 | 92% | 91.3% | (many diffs) |
| 4 | human | 9560 | CCL4L2 | C-C motif chemokine ligand ... | NM_001291473.2 | 75.5% | 61.3% | (many diffs) |
| 5 | human | 9560 | CCL4L2 | C-C motif chemokine ligand ... | NM_001291475.2 | 73.2% | 64% | (many diffs) |
| 6 | human | 9560 | CCL4L2 | C-C motif chemokine ligand ... | NM_001291472.2 | 72.1% | 62.2% | (many diffs) |
| 7 | human | 9560 | CCL4L2 | C-C motif chemokine ligand ... | NM_001291471.2 | 69.4% | 58.4% | (many diffs) |
| 8 | human | 9560 | CCL4L2 | C-C motif chemokine ligand ... | NM_001291469.2 | 68.1% | 67.3% | (many diffs) |
| 9 | human | 9560 | CCL4L2 | C-C motif chemokine ligand ... | NM_001291470.2 | 68.1% | 67.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 345
- ORF length:
- 276
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagctctgc gtgactgtcc tgtctctcct catgctagta gctgccttct 121 gctctccagc gctcTCAGCA CCAATGGGCT CAGACCCTCC CACCGCCTGC TGCTTTTCTT 181 ACACCGCGAG GAAGCTTCCT CGCAACTTTG TGGTAGATTA CTATGAGACC AGCAGCCTCT 241 GCTCCCAGCC AGCTGTGGTA TTCCAAACCA AAAGAAGCAA GCAAGTCTGT GCTGATCCCA 301 GTGAATCCTG GGTCCAGGAG TACGTGTATG ACCTGGAACT GAACTTGCCA ACTTTCTTGT 361 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 421 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 481 GAAAGGACGA AACGAATCGG CCCAGTCCGC GACAACGCGT TAAGTCgaca atcaacctct 541 ggattacaaa atttgtgaaa gatt