Construct: ORF TRCN0000477728
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010914.1_s317c1
- Derived from:
- ccsbBroadEn_04834
- DNA Barcode:
- ATGCCCTGAATGTTCTGGGTGTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LIX1L (128077)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477728
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 128077 | LIX1L | limb and CNS expressed 1 like | NM_153713.3 | 99.9% | 100% | 99C>T |
| 2 | human | 128077 | LIX1L | limb and CNS expressed 1 like | XM_017000316.1 | 90.4% | 90.5% | 99C>T;597_598ins96 |
| 3 | mouse | 280411 | Lix1l | Lix1-like | NM_001163170.1 | 90.4% | 97% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1077
- ORF length:
- 1011
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gactatgcga gcgcagcggc tgcagcctgg tgtgggcacc agcgggaggg 121 gcactctccg agcgctgcgg cccggagtga ctggggccgc ggctgccacc gccacacccc 181 ctgcgggccc cccgcctgcc ccgccgcctc ccgcaccgcc cccgccgccg ctgctcctgt 241 ctggggcccc aggactaccc ctgccccccg gcgccgccgg cagcccggca gtgctgcgag 301 aggccgtgga ggccgtggtg aggagcttcg ccaagcacac gcagggctat ggccgagtga 361 atgtggtgga ggcacttcag gaattctggc agatgaagca gtcccgtggt gctgacttaa 421 agaatggggc tctagtggtt tatgagatgg ttccctccaa cagccctcct tatgtctgct 481 atgtcaccct gcctggggga agctgctttg ggagtttcca gttttgcccc acaaaagctg 541 aggcccggag gagtgctgca aagattgcgc taatgaattc tgtgtttaat gaacatcctt 601 cccgaagaat cactgatgag ttcatcgaga agagtgtctc tgaggccctg gCATCTTTTA 661 ATGGCAACAG GGAGGAAGCT GACAACCCAA ATACAGGGAT TGGTGCCTTC CGATTCATGC 721 TGGAATCCAA CAAGGGCAAA TCAATGTTGG AGTTCCAGGA GCTAATGACA GTTTTTCAAC 781 TGCTACACTG GAATGGCAGC CTTAAGGCCA TGAGGGAACG ACAATGCTCT CGGCAGGAGG 841 TGTTGGCTCA TTATTCGCAC CGGGCCCTGG ATGATGATAT TCGCCACCAA ATGGCCTTGG 901 ACTGGGTGAG CCGGGAGCAG AGTGTGCCGG GGGCACTGTC TAGAGAGCTG GCCTCTACTG 961 AGCGGGAGCT GGATGAAGCC CGACTGGCAG GCAAGGAGCT GCGCTTCCAC AAGGAGAAGA 1021 AAGATATTCT TGTGCTGGCT GCTGGGCAGT TGGGCAATAT GCATTCTTCC AACTGCTACC 1081 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1141 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1201 ATATATCTTG TGGAAAGGAC GAATGCCCTG AATGTTCTGG GTGTGCACGC GTTAAGTCga 1261 caatcaacct ctggattaca aaatttgtga aagatt