Construct: ORF TRCN0000477730
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005206.1_s317c1
- Derived from:
- ccsbBroadEn_13011
- DNA Barcode:
- ATCCTCTGTGCCAAGCTCTGTCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EGLN3 (112399)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477730
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 112399 | EGLN3 | egl-9 family hypoxia induci... | NM_001308103.2 | 82% | 82% | 1_78del |
| 2 | human | 112399 | EGLN3 | egl-9 family hypoxia induci... | NM_022073.4 | 49.7% | 49.7% | 1_360del |
| 3 | mouse | 112407 | Egln3 | egl-9 family hypoxia-induci... | NM_028133.2 | 44.6% | 48.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 450
- ORF length:
- 357
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGGTGGC TTGCTATCCG GGAAATGGAA 121 CAGGTTATGT TCGCCACGTG GACAACCCCA ACGGTGATGG TCGCTGCATC ACCTGCATCT 181 ACTATCTGAA CAAGAATTGG GATGCCAAGC TACATGGTGG GATCCTGCGG ATATTTCCAG 241 AGGGGAAATC ATTCATAGCA GATGTGGAGC CCATTTTTGA CAGACTCCTG TTCTTCTGGT 301 CAGATCGTAG GAACCCACAC GAAGTGCAGC CCTCTTACGC AACCAGATAT GCTATGACTG 361 TCTGGTACTT TGATGCTGAA GAAAGGGCAG AAGCCAAAAA GAAATTCAGG AATTTAACTA 421 GGAAAACTGA ATCTGCCCTC ACTGAAGACT TGCCAACTTT CTTGTACAAA GTGGTTGATA 481 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 541 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAATCCT 601 CTGTGCCAAG CTCTGTCCCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 661 tgaaagatt