Construct: ORF TRCN0000477746
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013796.1_s317c1
- Derived from:
- ccsbBroadEn_13282
- DNA Barcode:
- TAAGAATTATATATTCTGTGGACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPP4R2 (151987)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477746
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 151987 | PPP4R2 | protein phosphatase 4 regul... | NM_001318025.2 | 100% | 100% | |
| 2 | human | 151987 | PPP4R2 | protein phosphatase 4 regul... | NM_001318026.1 | 87.4% | 87.6% | (many diffs) |
| 3 | human | 151987 | PPP4R2 | protein phosphatase 4 regul... | NM_174907.4 | 86.3% | 86% | 117_287del |
| 4 | human | 151987 | PPP4R2 | protein phosphatase 4 regul... | NM_001318027.2 | 68.3% | 63.4% | 0_1ins280;41_42ins62 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1149
- ORF length:
- 1080
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggacgtcgag aggctccagg aggcgctgaa agattttgag aagaggggga 121 aaaaggaagt ttgtcctgtc ctggatcagt ttctttgtca tgtagccaag actggagaaa 181 caattatccc ttttactatt cagcgactat gtgaattgtt aacagatcca aggagaaact 241 atacaggaac agacaaattt ctcagaggag tagaaaagaa tgtgatggtt gttagctgtg 301 tttatccttc ttcagagaaa aacaattcca atagtttaaa tcgaatgaat ggtgttatgt 361 ttcctggaaa ttcaccaagc tatactgaga ggtctaatat aaatgggcct gggacaccca 421 ggccacttaa tcgaccaaag gtttctttgt cagcccccat gacaacaaat gggttgcctg 481 agagcacaga cagcaaagag gcaaatttgc agcaaaatga ggagaaaaat cacagtgact 541 cttcgacctc tgaatcagaa gtttcctcag tgagcccttt gaaaaataaa catccagatg 601 aagatgctgt ggaagctgag gggcatgagg taaaaagact caggtttgac aaagaaggtg 661 aagtcagaga aacagccagt caaacgactt ccagcgaaat ttcttcagtt atggtaggag 721 aaacagaagc atcatcttca TCTCAGGATA AAGACAAAGA TAGCCGTTGT ACCCGGCAGC 781 ACTGTACAGA AGAGGATGAA GAAGAGGATG AAGAGGAAGA AGAAGAGTCT TTTATGACAT 841 CAAGAGAAAT GATCCCAGAA AGAAAAAATC AAGAAAAAGA ATCTGATGAT GCCTTAACTG 901 TGAATGAAGA GACTTCTGAG GAAAATAATC AAATGGAGGA ATCTGATGTG TCTCAAGCTG 961 AGAAAGATTT GCTACATTCT GAAGGTAGTG AAAACGAAGG CCCTGTAAGT AGTAGTTCTT 1021 CTGACTGCCG TGAAACAGAA GAATTAGTAG GATCCAATTC CAGTAAAACT GGAGAGATTC 1081 TTTCAGAATC ATCCATGGAA AATGATGACG AAGCCACAGA AGTCACCGAT GAACCAATGG 1141 AACAAGACTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1201 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1261 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATAAGAA TTATATATTC TGTGGACAAC 1321 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt