Construct: ORF TRCN0000477749
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005954.1_s317c1
- Derived from:
- ccsbBroadEn_10194
- DNA Barcode:
- CTTAGGCCCTTACCGTTGCAACCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GSTT2B (653689)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477749
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 653689 | GSTT2B | glutathione S-transferase t... | NM_001080843.3 | 99.8% | 100% | 363A>C |
| 2 | human | 2953 | GSTT2 | glutathione S-transferase t... | NM_000854.4 | 99.7% | 99.5% | 363A>C;385A>G |
| 3 | human | 653689 | GSTT2B | glutathione S-transferase t... | NM_001363804.1 | 94.1% | 94.2% | 363A>C;479_480ins42 |
| 4 | human | 2953 | GSTT2 | glutathione S-transferase t... | NM_001302670.1 | 93.9% | 93.8% | 363A>C;385A>G;479_480ins42 |
| 5 | human | 2953 | GSTT2 | glutathione S-transferase t... | NR_126445.2 | 65.7% | (many diffs) | |
| 6 | human | 2953 | GSTT2 | glutathione S-transferase t... | NR_126446.1 | 51.5% | (many diffs) | |
| 7 | human | 2953 | GSTT2 | glutathione S-transferase t... | NR_126442.1 | 46.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 801
- ORF length:
- 732
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggcctagag ctgtttcttg acctggtgtc ccagcccagc cgcgccgtct 121 acatcttcgc caagaagaat ggcatcccct tagagctgcg caccgtggat ttggtcaaag 181 ggcagcacaa gagcaaggag ttcttgcaga tcaacagcct ggggaaactg ccgacgctca 241 aggatggtga tttcatcttg accgaaagct cggccatcct gatttacctg agctgtaagt 301 accagacgcc ggaccactgg tatccatctg acctgcaggc tcgtgcccgt gttcatgagt 361 acctgggctg gcatgccgac tgcatccgtg gcacctttgg tatacccctg tgggtccagg 421 tgttggggcc cctcattggg gtccaggtgc ccgaggagaa ggtggaacgc aacaggactg 481 ccatggacca ggccctgcaa tggctggagg acaagttccT GGGGGACAGG CCCTTCCTCG 541 CTGGCCAGCA GGTGACACTG GCTGATCTCA TGGCCCTGGA GGAGCTGATG CAGCCGGTGG 601 CTCTCGGCTA TGAACTGTTT GAGGGACGGC CACGACTGGC AGCATGGCGT GGACGAGTGG 661 AGGCTTTCCT GGGTGCTGAG CTATGCCAGG AGGCCCACAG CATCATCTTG AGCATCCTGG 721 AACAGGCGGC CAAGAAAACC CTCCCAACAC CCTCACCAGA GGCCTATCAG GCTATGCTGC 781 TTCGAATCGC CAGGATCCCC TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 841 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 901 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACTTA GGCCCTTACC 961 GTTGCAACCC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt