Construct: ORF TRCN0000477837
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016440.1_s317c1
- Derived from:
- ccsbBroadEn_11584
- DNA Barcode:
- TTACGCATGAGGACGAACTTATCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UPK1A (11045)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477837
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11045 | UPK1A | uroplakin 1A | NM_001281443.1 | 100% | 100% | |
| 2 | human | 11045 | UPK1A | uroplakin 1A | NM_007000.3 | 82.6% | 75.9% | 646_647ins98;722_774del |
| 3 | mouse | 109637 | Upk1a | uroplakin 1A | NM_026815.2 | 72.3% | 70.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 888
- ORF length:
- 819
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgtctgcg gcagcagcgg aggccgagaa gggatctcca gttgtggtgg 121 gcctgctagt tgtgggcaat atcattattc tgctgtcagg cctgtccctg tttgctgaga 181 ccatatgggt gacagccgac cagtaccgtg tatacccact gatgggagtc tcaggcaagg 241 atgacgtctt cgctggtgcc tggattgcca tcttctgcgg cttctccttc ttcatggtag 301 ccagttttgg tgtgggtgcc gcactctgcc gccgccggtc catggtcctc acgtacctgg 361 tgctcatgct catcgtctac atcttcgagt gcgcctcctg catcacgtcc tacacccacc 421 gtgactacat ggtgtccaac ccatccctga tcaccaagca gatgctgacc ttcTACAGCG 481 CGGACACCGA CCAGGGCCAG GAGCTGACCC GCCTCTGGGA CCGCGTCATG ATTGAGCAAG 541 AATGCTGTGG CACATCTGGT CCCATGGACT GGGTGAACTT CACGTCAGCC TTCCGGGCGG 601 CCACTCCGGA GGTGGTGTTC CCCTGGCCCC CACTGTGCTG TCGCCGGACG GGAAACTTCA 661 TCCCCCTCAA CGAGGAGGGC TGCCGCCTGG GGCACATGGA CTACCTGTTC ACCAAGGCTG 721 GAGTACAGTG GCACAATCTC AGCTCACTGC AGCGTCTGCC TCCCGGGTTC AAGGGATTCT 781 CCCACCTCAG CTTCCAGAGC AGCTGGGATT ACAGGGCTGC TTCGAACACA TCGGCCACGC 841 CATCGACAGC TACACGTGGG GTATCTCGTG GTTTGGGTTT GCCATCCTTG CCAACTTTCT 901 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 961 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1021 GTGGAAAGGA CGATTACGCA TGAGGACGAA CTTATCAACG CGTTAAGTCg acaatcaacc 1081 tctggattac aaaatttgtg aaagatt