Construct: ORF TRCN0000477912
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002319.1_s317c1
- Derived from:
- ccsbBroadEn_08545
- DNA Barcode:
- GATCGTACTCAGGTCGTTGCCGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CNOT11 (55571)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477912
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55571 | CNOT11 | CCR4-NOT transcription comp... | NM_017546.5 | 99.8% | 99.8% | 162G>T;1519A>G |
| 2 | mouse | 52846 | Cnot11 | CCR4-NOT transcription comp... | NM_028043.2 | 89.5% | 95.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1596
- ORF length:
- 1530
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc cggcggaggg gcgagcgcgg cgtctggccg gcttctcacc gccgcggagc 121 aaagagggtc ccgggaagcg gcagggtcgg cgtccaggag cggcttcggg ggctccggcg 181 gcggcagagg cggagcaagc ggccccgggt ccgggagcgg aggccctggg ggccccgcgg 241 gcaggatgag cttgaccccg aaggagctct cgagcctgct gagcatcata tcggaggagg 301 cgggcggcgg cagcaccttc gagggcctgt ccaccgcctt ccaccactac ttcagcaagg 361 ccgaccactt ccgcctgggc tcggtgctcg tcatgctgct ccagcagccc gacctgctgc 421 ctagcgcggc gcagcgcctc acggcgctct acctgctctg ggagatgtac cgcaccgagc 481 cgctggccgc caaccccttc gccgccagct tcgcgcacct gctcaacccc gcgccgcccg 541 cccgcggcgg ccaggaaccc gaccgccctc cgctctcagg atttttacct cctataactc 601 caccagaaaa gttttttctt tcccagctga tgctggcacc cccacgggaa ctcttcaaaa 661 agacgcctcg ccagattgca ctgatggacg ttggaaacat gggccagtct gtggacatta 721 gtgggcttca gttagccttg gccgaacgcc aatctgaatt gccaacgcaa agcaaagcga 781 gcttccccag tattctcagt gacccagacc cggattcttc taattctgga tttgacagct 841 cagttgcctc tcagatcaca gaagctttag tcagcggacc aaagccacct attgaaagcc 901 attttcgacc agagtttatt cgtccaccgc ctccactcca catttgtgag gatgaacttg 961 cttggctaaa ccccacggag cctgaccacg cgatccagtg ggataaatcg atgtgtgtta 1021 agaatagcac tggtgtggag atcaaacgaa taatggccaa agccttcaaa agccccttat 1081 cctctcccca acaaacacag ctacttggtg agttggaaaa agaccccaaa cttgtctacc 1141 atattggcct caccccagcc aaacttcctg accttgtgga aaacaaccct ttagtcgcta 1201 tagaaatgtt gctgaaatta atgcagtcaa gccagatcac tgagtatttc tctgtcctgg 1261 tcaatatgga catgtcttta catTCAATGG AAGTTGTAAA TCGACTAACT ACAGCTGTTG 1321 ATCTACCTCC TGAATTTATT CACCTTTATA TATCAAATTG CATCTCTACT TGTGAACAGA 1381 TTAAGGATAA ATATATGCAG AATCGGTTGG TGCGTCTTGT GTGTGTGTTT CTCCAATCCT 1441 TGATCCGTAA CAAAATTATT AATGTACAGG ATTTGTTTAT AGAAGTGCAG GCATTCTGTA 1501 TTGAATTCAG TAGGATACGA GAAGCTGCTG GTCTTTTCCG GTTGTTGAAG ACATTGGATA 1561 CTGGGGAAAC ACCTTCTGAG ACCGAAATGT CAAAATACCC AACTTTCTTG TACAAAGTGG 1621 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1681 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1741 AGATCGTACT CAGGTCGTTG CCGGTACGCG TTAAGTCgac aatcaacctc tggattacaa 1801 aatttgtgaa agatt