Construct: ORF TRCN0000477921
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017737.1_s317c1
- Derived from:
- ccsbBroadEn_09124
- DNA Barcode:
- AGTCCACTCTTGCGTGTATCGCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TBC1D10A (83874)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477921
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83874 | TBC1D10A | TBC1 domain family member 10A | NM_031937.3 | 99.8% | 100% | 444C>A;807C>T |
| 2 | human | 83874 | TBC1D10A | TBC1 domain family member 10A | NM_001204240.2 | 98.5% | 98.6% | 210_230del;465C>A;828C>T |
| 3 | mouse | 103724 | Tbc1d10a | TBC1 domain family, member 10a | NM_134023.1 | 86.7% | 90.7% | (many diffs) |
| 4 | mouse | 103724 | Tbc1d10a | TBC1 domain family, member 10a | XM_006514441.2 | 70.9% | 74.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1593
- ORF length:
- 1524
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgaagagc aacggagaga atgggccgcg cgcgcccgcg gccggggaaa 121 gcctgtcggg aacccgggag agcctggccc agggccccga cgccgcaacc accgacgaac 181 tcagctctct cgggtctgac tcggaggcca acggcttcgc cgagcgccgc atcgacaagt 241 tcggcttcat cgtgggctcg cagggcgccg agggcgcgct ggaggaagta cccctggagg 301 tgctgaggca gagggagtcc aagtggctgg acatgctcaa caactgggac aaatggatgg 361 ccaagaagca caaaaagatt cgtctgcggt gccaaaaggg catcccgcct tctctgcggg 421 gccgtgcttg gcagtacctg tcaggaggca aggtgaagtt acagcagaac cctggaaagt 481 ttgacgagct ggacatgtcc cctggggacc caaagtggct ggacgtgatt gagcgtgacc 541 tgcaccggca gttcccattc catgagatgt ttgtgtcccg ggggggccac ggccagcagg 601 acctattccg tgtgctgaag gcctacacgc tgtaccggcc cgaggagggc tactgccagg 661 cccaggcgcc cattgccgct gtcttgctca tgcatatgcc tgctgagcaa gccttctggt 721 gcctggtaca gatctgtgag aagtacctgc ccggctacta cagcgagaaa ctggaggcga 781 tccagctgga cggggagatc cttttctcgc tgttgcagaa ggtgtcgccg gtggcccaca 841 agcacctcag ccgtcagaag atcgacccgc tcctttatat gacagaatgg ttcatgtgcg 901 ccttctcccg aaccttgccc tggagctctg tgctgcgtgt ctgggacatg ttcttctgtg 961 aaggggtcaa gatcatcttc cgggtggggc tggtgctgct gaagcacgcg ctgggctccc 1021 ctgagaaggt caaagcctgc cagggccagt acgagaccat cgagcgactg cggagcctca 1081 gccccaagat catgcaggag gcctttctgg tccaggaggt ggtggagttg cccgtgacag 1141 agcgccagat tgagcgcgaa cacctcattc agctgcggcg ctggcaggag acccggggtg 1201 agctgcagtg ccgctccccg cccaggctgc atggtgccaa ggctatcttg gatgcagaac 1261 ctggTCCCCG GCCTGCCCTA CAACCTTCAC CATCCATCCG CCTGCCCCTA GATGCCCCCC 1321 TCCCTGGCTC CAAAGCCAAG CCCAAGCCAC CCAAGCAGGC CCAGAAGGAG CAGCGGAAAC 1381 AGATGAAGGG GAGAGGGCAG CTGGAGAAGC CCCCAGCCCC AAATCAAGCC ATGGTGGTGG 1441 CCGCTGCAGG AGATGCATGT CCCCCACAGC ATGTGCCCCC GAAGGACTCA GCCCCCAAGG 1501 ACTCAGCCCC TCAGGATTTG GCTCCCCAGG TCTCAGCCCA CCACCGCTCC CAGGAGAGCT 1561 TGACGTCCCA AGAGAGTGAG GACACCTACT TGTTGCCAAC TTTCTTGTAC AAAGTGGTTG 1621 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1681 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAG 1741 TCCACTCTTG CGTGTATCGC CGACGCGTTA AGTCgacaat caacctctgg attacaaaat 1801 ttgtgaaaga tt