Construct: ORF TRCN0000477933
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015212.1_s317c1
- Derived from:
- ccsbBroadEn_05624
- DNA Barcode:
- ATTCTTAGATATACTTAATACCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OR2A25 (392138)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477933
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 392138 | OR2A25 | olfactory receptor family 2... | NM_001004488.1 | 100% | 100% | |
| 2 | human | 401427 | OR2A7 | olfactory receptor family 2... | NM_001005328.1 | 83% | 79.6% | (many diffs) |
| 3 | human | 107987545 | LOC107987545 | olfactory receptor 2A7 | XM_024452660.1 | 83% | 79.6% | (many diffs) |
| 4 | human | 79541 | OR2A4 | olfactory receptor family 2... | NM_030908.2 | 82.6% | 79% | (many diffs) |
| 5 | human | 728377 | ARHGEF34P | Rho guanine nucleotide exch... | NR_033942.1 | 12.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 999
- ORF length:
- 930
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggggggaaat cagacttcca tcacagagtt cctcctactg ggatttccca 121 ttggcccaag gattcagatg ctcctctttg ggctcttctc cctgttctac atcttcattc 181 tgttggggaa cgggacaatc ctggggctca tctcactgga ctccagactc cacaccccca 241 tgtacttctt cctctcacac ctggcggtcg tcgacatcgc ctgtgcttgc agcacggtgc 301 cccagatgct ggtgaacctc ctgcatccag ccaagcccat ctcctttgct ggctgcatga 361 cccagatgtt tctgtttttg agttttgcac atacagaatg tctcctcctg gtggtgatgt 421 cctatgatcg gtacgtggcc atctgccacc ctctccgata ttctaccatc atgacctgga 481 aagtctgcat cactttggca ttgacttcct ggattttagg agtcttattg gcccttgtcc 541 atctagtgtt actgctacca ctgtccttct gtggacccca gaaacttaat cactttttct 601 gtgaaattat ggctgttctc aaacttgcct gtgcggatac ccacattaat gaggtaatgg 661 ttttggcagg ggcagtgtct gtgctggtgg gagccttctt ttccactgta atatcttatg 721 ttcatattct atgtgccatT CTAAAGATCC AGTCAGGAGA GGGGTGCCAG AAAGCCTTCT 781 CCATCTGCTC CTCCCACCTC TGTGTGGTTG GACTCTTTTA TGGCACAGCC ATCATCATGT 841 ATGTTGAGCC CCAGTATGAG AGCCCCAAGG AGCAGAAGAA ATATCTCCTG CTGTTTCACA 901 GCCTCTTCAA TCCCATGCTT AATCCCCTAA TTTATAGTCT TAGGAACAAG GAAGTCCAAG 961 GTACTCTAAA GAGGATGCTT GAAAAGAAGA GAACTTCATT GCCAACTTTC TTGTACAAAG 1021 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1081 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1141 ACGAATTCTT AGATATACTT AATACCAAAC GCGTTAAGTC gacaatcaac ctctggatta 1201 caaaatttgt gaaagatt