Construct: ORF TRCN0000477966
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000673.1_s317c1
- Derived from:
- ccsbBroadEn_14303
- DNA Barcode:
- CCTATCTTGCAGGGCTTACGAATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ID2B (84099)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477966
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3398 | ID2 | inhibitor of DNA binding 2 | NM_002166.5 | 26.3% | 26.1% | 95C>T;108_402delinsG |
| 2 | mouse | 15902 | Id2 | inhibitor of DNA binding 2 | NM_010496.3 | 25.3% | 26.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 240
- ORF end:
- 348
- ORF length:
- 108
- Sequence:
-
1 GTCTTTAGGT TGGGGGGAGG GGTTTTATGC GATGGAGTTT CCCCACACTG AGTGGGTGGA 61 GACTGAAGTT AGGCCAGCTT GGCACTTGAT GTAATTCTCC TTGGAATTTG CCCTTTTTGA 121 GTTTGGATCT TGGTTCATTC TCAAGCCTCA GACAGTGGTT CAAAGTTTTT TTCTTCCATT 181 TCAGGTGTCG TGAGGCTAGC ATCGATTGAT CAACAAGTTT GTACAAAAAA GTTGGCACCA 241 TGAAAGCCTT CAGTCCCGTG AGGTCCGTTA GGAAAAACAG CCTGTCGGAC CACAGCCTGG 301 GCATCTCCCG GAGCAAAACC CCTGTGGACG ACCTGATGAG CCTGCTGTTG CCAACTTTCT 361 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 421 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 481 GTGGAAAGGA CGACCTATCT TGCAGGGCTT ACGAATTACG CGTTAAGTCg acaatcaacc 541 tctggattac aaaatttgtg aaagatt