Construct: ORF TRCN0000477970
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004398.1_s317c1
- Derived from:
- ccsbBroadEn_13208
- DNA Barcode:
- TGATACGCGCCGTTAATCCAATGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C20orf173 (140873)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477970
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 140873 | C20orf173 | chromosome 20 open reading ... | XM_024451835.1 | 74.7% | 62.2% | (many diffs) |
| 2 | human | 140873 | C20orf173 | chromosome 20 open reading ... | NM_001145350.2 | 65% | 52.3% | (many diffs) |
| 3 | human | 140873 | C20orf173 | chromosome 20 open reading ... | XM_006723710.2 | 62.3% | 48.8% | (many diffs) |
| 4 | human | 140873 | C20orf173 | chromosome 20 open reading ... | NR_026933.2 | 19.7% | (many diffs) | |
| 5 | human | 140873 | C20orf173 | chromosome 20 open reading ... | XR_936504.1 | 16.9% | (many diffs) | |
| 6 | human | 140873 | C20orf173 | chromosome 20 open reading ... | XR_001754182.1 | 16.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 516
- ORF length:
- 447
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctttccggg cctcacccat ctcccacttt ccgcccaaat ccctgccctt 121 ggccctGTCT TCACAGCCTC TGGATGGAGA TCTCACCCAC CCAGCTCTGT TTTCTCTCCC 181 CTGGGCCCAG CCCCCAATCT CCCTCTTGCT GTTTCCAGGG CATGAACTCA GGGAGCGAGC 241 TTGGGAAATT GTGGAGGAAG CTGTTTAAAG GGATTCCCAG GCTCTCGGTG AGCCATTTTG 301 ATTTCTATTG TGGGACTTGT GTGCTGTTGG GGCGCCCACA GATCCCGCAG GGCTCCAGCC 361 TTGGCAACGA CATCGACCAA TACCCCGTGG TTTTCAGGAA TGCCAGCGAC CAGGGCTCCT 421 GGATGCAGCT GGAGATGCTA CTGCGGAAGC TCTCTGACCT GGTGTGGACT TCAGATGCTC 481 TAAGTGATAA GATTTTGGAA GATGGTCTGG TTCCCTTGCC AACTTTCTTG TACAAAGTGG 541 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 601 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 661 ATGATACGCG CCGTTAATCC AATGAACGCG TTAAGTCgac aatcaacctc tggattacaa 721 aatttgtgaa agatt