Construct: ORF TRCN0000477998
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002363.1_s317c1
- Derived from:
- ccsbBroadEn_07427
- DNA Barcode:
- CCAACGTGTATCGCGAAAGGCTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PIGB (9488)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477998
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9488 | PIGB | phosphatidylinositol glycan... | NM_004855.5 | 99.7% | 99.4% | (many diffs) |
| 2 | human | 9488 | PIGB | phosphatidylinositol glycan... | XM_011522235.3 | 97% | 96.7% | (many diffs) |
| 3 | human | 9488 | PIGB | phosphatidylinositol glycan... | XM_011522236.3 | 93.4% | 93.1% | (many diffs) |
| 4 | human | 9488 | PIGB | phosphatidylinositol glycan... | XM_011522237.2 | 75.8% | 74.9% | (many diffs) |
| 5 | human | 9488 | PIGB | phosphatidylinositol glycan... | XM_017022730.1 | 75.8% | 74.9% | (many diffs) |
| 6 | human | 9488 | PIGB | phosphatidylinositol glycan... | XM_017022731.1 | 75.8% | 74.9% | (many diffs) |
| 7 | human | 9488 | PIGB | phosphatidylinositol glycan... | XM_005254795.5 | 70.7% | 70.5% | (many diffs) |
| 8 | human | 9488 | PIGB | phosphatidylinositol glycan... | XM_017022732.2 | 68% | 67.8% | (many diffs) |
| 9 | human | 9488 | PIGB | phosphatidylinositol glycan... | XR_931950.3 | 65.8% | (many diffs) | |
| 10 | human | 9488 | PIGB | phosphatidylinositol glycan... | XR_001751422.2 | 65.4% | (many diffs) | |
| 11 | human | 9488 | PIGB | phosphatidylinositol glycan... | XR_001751423.2 | 63.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1728
- ORF length:
- 1662
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gaggccccta agcaagtgcg gaatggagcc ggggggcgga gatgccagcc 121 tcactttgca tggtctccag aaccgctccc acggcaagat aaagctgcga aagagaaagt 181 ctaccttgta cttcaacacc caggagaaga gcgccaggcg ccgcggggat cttcttggag 241 aaaatattta tctgctcttg tttaccttag ctttacgaat attaaactgc tttttagtgc 301 agacaagttt tgttccagat gaatactggc agtctcttga agtttcacat cacatggttt 361 tcaattatgg ttatttgact tgggaatgga cagagagact gaggagttac acttatccct 421 taatctttgc aagcatttac aagattcttc atcttttagg gaaagatagt gttcagttgc 481 tgatttggat tcctagactt gcccaagcac ttctgtctgc tgtagcagat gtgagacttt 541 actcattaat gaagcaacta gaaaatcagg aagtggcaag atgggtgttt ttttgccagt 601 tgtgctcctg gttcacatgg tattgctgta ccagaaccct tacaaacacc atggaaactg 661 ttctcactat aattgctctt ttctactatc ctttggaagg ttcaaagtct atgaacagtg 721 tcaaatactc atccctggtg gcacttgcct tcataattcg tcccacagct gtcattctgt 781 ggacaccttt gctcttcaga catttctgtc aagaaccaag aaagcttgat cttattctac 841 atcatttttt acctgtaggc tttgttactt tgagtttgtc tctgatgatt gatcgtattt 901 tttttggcca atggactctg gttcaattta attttttgaa atttaacgtg ctgcagaact 961 tgggaacatt ttatggttct catccatggc actggtactt cagtcaagga tttccagtta 1021 tcttgggtac tcacttaccc ttctttattc atggctgcta tctagcacca aagagatacc 1081 ggatactttt ggtgactgtg ctgtggacac tgcttgttta tagcatgttg agccacaaag 1141 aattcaggtt tatttatcca gttttaccat tctgtatggt gttctgtgga tactcattaa 1201 cccacctgaa aacatggaag aaaccagctc taagtttcct gtttttatca aatttgttcc 1261 tcgcccttta tactggttta gttcatcaac gaggtactct tgatgtcatg agtcatattc 1321 aaaaagtttg ttaCAACAAT CCCAATAAAT CTTCAGCTTC AATATTTATA ATGATGCCTT 1381 GCCACTCTAC TCCTTATTAC AGCCATGTTC ACTGCCCACT TCCCATGAGA TTTCTCCAGT 1441 GCCCGCCAGA CCTGACTGGA AAAAGTCATT ATCTTGATGA AGCAGATGTA TTTTACCTAA 1501 ATCCCTTAAA CTGGTCACAT AGAGAGTTTC ATGATGATGC ATCATTGCCT ACTCACTTGA 1561 TCACCTTCAG CATTTTGGAA GAGGAAATAA GTGCTTTCCT AATTTCAAGC AATTATAAAA 1621 GAACTGCTGT TTTCTTCCAC ACTCACTTGC CAGAGGGTCG AATTGGAAGT CACATATATG 1681 TCTATGAACG GAAGTTAAAA GGGAAATTCA ACATGAAGAT GAAATTCTGC CCAACTTTCT 1741 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1801 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1861 GTGGAAAGGA CGACCAACGT GTATCGCGAA AGGCTGAACG CGTTAAGTCg acaatcaacc 1921 tctggattac aaaatttgtg aaagatt