Construct: ORF TRCN0000478028
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017982.1_s317c1
- Derived from:
- ccsbBroadEn_04811
- DNA Barcode:
- CCCGGAGACGGTTGACGTGTGTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EID2B (126272)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478028
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 126272 | EID2B | EP300 interacting inhibitor... | NM_152361.3 | 99.7% | 100% | 135T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 552
- ORF length:
- 483
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggagccg actgggctgt tggagatgtc cgagctcccc ggagatagca 121 gtgtcccaca ggtgggcacc gcgagtggcg tcagcgacgt actgcggggg gcagtcggcg 181 gcggggttcg ggtgcaggag gcccgggaag gcccagtggc cGAAGCTGCG CGGTCCATGG 241 CGCGGATGCC GGGCCCTGTG CCCGGGCCCA TCCCCAGCAG CGTCCCGGGC CTGGCCTCCG 301 CGCCAGACCC CCATCAGCAG CTCGCTTTCT TAGAAATTAA CCGGCAGCTG TTGTTCCGCG 361 AGTATCTGGA TGGTAGCTCC ATGATTCCGG TCAGATTACT ACGGGATTTC GAGGAACGCC 421 GCAGGCTGTT TGTGGAAGGC TGCAAGGCGA GGGAAGCAGC CTTTGACGCG GATCCCCCGC 481 AGATGGACTT CGCTGCTGTC GCGTTTACGG TAGCGCTGAC TGCCTCCGAG GCCCTCAGTC 541 CTCTGGCCGA CTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 601 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 661 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACCC GGAGACGGTT GACGTGTGTA 721 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t