Construct: ORF TRCN0000478081
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011201.1_s317c1
- Derived from:
- ccsbBroadEn_05637
- DNA Barcode:
- TGTCCCCATCACCATGAGTGCCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VMAC (400673)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478081
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 400673 | VMAC | vimentin type intermediate ... | NM_001017921.4 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 576
- ORF length:
- 507
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcggcgccg ccggccctgc agatccggga ggcaaacgca cacctggcag 121 ccgtgcaccg gcgcgcagcg gagctggagg cgcggctgga cgcggcggag cgcacggtgc 181 acgcccaagc cgagcgcctg gccctccacg acCAGCAGCT GCGCGCCGCC CTAGACGAAC 241 TGGGTCGCGC CAAGGACCGT GAGATTGCCA CACTCCAGGA GCAGCTGATG ACCTCAGAAG 301 CCACTGTCCA CAGCCTGCAG GCCACCGTGC ACCAGAGGGA CGAGCTCATT AGGCAGTTGC 361 AGCCCCGGGC TGAGCTGCTG CAGGACATCT GCCGCCGCCG GCCACCCCTG GCTGGGCTGC 421 TGGATGCCCT GGCTGAGGCT GAGCGCCTGG GGCCCCTGCC GGCCAGTGAC CCCGGCCACC 481 CACCCCCCGG TGGGCCTGGT CCACCCCTTG ACAACAGCAC TGGGGAAGAG GCGGACAGGG 541 ACCACCTCCA GCCTGCAGTG TTTGGGACCA CAGTGTTGCC AACTTTCTTG TACAAAGTGG 601 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 661 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 721 ATGTCCCCAT CACCATGAGT GCCGTACGCG TTAAGTCgac aatcaacctc tggattacaa 781 aatttgtgaa agatt