Construct: ORF TRCN0000478137
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016671.1_s317c1
- Derived from:
- ccsbBroadEn_05503
- DNA Barcode:
- CACGCCGCATAAGATGTGCACGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OR2A12 (346525)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478137
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 346525 | OR2A12 | olfactory receptor family 2... | NM_001004135.1 | 100% | 100% | |
| 2 | mouse | 258292 | Olfr446 | olfactory receptor 446 | NM_146295.1 | 81.3% | 84.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 999
- ORF length:
- 930
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaaagcaat cagacctgga tcacagaagt catcctgttg ggattccagg 121 tggacccagc tctggagttg ttcctctttg ggtttttctt gctattctac agcttaaccc 181 tgatgggaaa tgggattatc ctggggctca tctacttgga ctctagactg cacacaccca 241 tgtatgtctt cctgtcacac ctggccattg tggacatgtc ctatgcctcg agtactgtcc 301 ctaagatgct agcaaatctt gtgatgcaca aaaaagtcat ctcctttgct ccttgcatac 361 ttcagacttt tttgtatttg gcgtttgcta ttacagagtg tctgattttg gtgatgatgt 421 gctatgatcg gtatgtggca atctgtcacc ccttgcaata caccctcatt atgaactgga 481 gagtgtgcac tgtcctggcc tcaacttgct ggatatttag ctttctcttg gctctggtcc 541 atattactct tattctgagg ctgccttttt gtggcccaca aaagatcaac cactttttct 601 gtcaaatcat gtccgtattc aaattggcct gtgctgacac taggctcaac caggtggtcc 661 tatttgcggg ttctgcgttc atctTAGTGG GGCCGCTCTG CCTGGTGCTG GTCTCCTACT 721 TGCACATCCT GGTGGCCATC TTGAGGATCC AGTCTGGGGA GGGCCGCAGA AAGGCCTTCT 781 CTACCTGCTC CTCCCACCTC TGCGTGGTGG GGCTTTTCTT TGGCAGCGCC ATTGTCATGT 841 ACATGGCCCC CAAGTCAAGC CATTCTCAAG AACGGAGGAA GATCCTTTCC CTGTTTTACA 901 GCCTTTTCAA CCCGATCCTG AACCCCCTCA TCTACAGCCT TAGGAATGCA GAGGTGAAAG 961 GGGCTCTAAA GAGAGTCCTT TGGAAACAGA GATCAATGTT GCCAACTTTC TTGTACAAAG 1021 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1081 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1141 ACGACACGCC GCATAAGATG TGCACGCAAC GCGTTAAGTC gacaatcaac ctctggatta 1201 caaaatttgt gaaagatt