Construct: ORF TRCN0000478188
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010756.1_s317c1
- Derived from:
- ccsbBroadEn_13420
- DNA Barcode:
- GGCTCAGTTTGTCACTAGAGCGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALKBH3 (221120)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478188
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 221120 | ALKBH3 | alkB homolog 3, alpha-ketog... | NM_139178.4 | 53.9% | 42.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 576
- ORF length:
- 510
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggaaaaaaga cggcgagccc gagttcaggg agcctgggct gcccctgtta 121 aaagccaggc cattgctcag ccagctacca ctgctaagag ccatctccac cagaagcctg 181 gccagacctg gaagaacaaa gagcatcatc tctctgacag agagtttgtg ttcaaagaac 241 ctcagcaggt agtacgtaga gctccTGAGC CACGAGTGAT TGAAGAGGGT GTGTATGAAA 301 TCAGCCTGTC ACCCACAGGT GTATCTAGGG TCTGTTTGTA TCCTGGCTTT GTTGACGTGA 361 AAGAAGCTGA CTGGATATTG GAACAGCTTT GTCAAGATGT TCCCTGGAAA CAGAGGACCG 421 GCATCAGAGA GGATTCCATA TTGCAACTAA CCTTTAAGAA GTCAGCACCT GTTAGTGGAA 481 CCGCGACTGC TCCGCAGAGC TGCTGGTATG AGCGCCCGTC GCCACCCCAC ATCCCAGGCC 541 CAGCCATTCT GACAAGAACT AGACTTTGGG CTCCTTGCCC AACTTTCTTG TACAAAGTGG 601 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 661 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 721 AGGCTCAGTT TGTCACTAGA GCGAGACGCG TTAAGTCgac aatcaacctc tggattacaa 781 aatttgtgaa agatt