Construct: ORF TRCN0000478289
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005054.2_s317c1
- Derived from:
- ccsbBroadEn_02848
- DNA Barcode:
- CGTCCTTCGTGCGATCCATGGAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HYPK (25764)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478289
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25764 | HYPK | huntingtin interacting prot... | NM_016400.4 | 93.7% | 93.7% | 0_1ins24 |
| 2 | human | 25764 | HYPK | huntingtin interacting prot... | NM_001199885.1 | 62.7% | 50.3% | 184_185ins56;243_244ins88 |
| 3 | human | 100529067 | SERF2-C15ORF63 | SERF2-C15orf63 readthrough | NR_037673.1 | 21.5% | 1_621del;1009_1794del | |
| 4 | mouse | 67693 | Hypk | huntingtin interacting prot... | NM_026318.3 | 87.3% | 99.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 453
- ORF length:
- 387
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg gcggcgtggt gaaatagata tggcgaccga gggggatgtg gagctggagt 121 tggagactga gaccagtgga ccagagcggc cTCCGGAGAA GCCACGGAAA CATGACAGCG 181 GTGCGGCGGA CTTGGAGCGG GTCACCGACT ATGCAGAGGA GAAGGAGATC CAGAGTTCCA 241 ATCTGGAGAC GGCCATGTCT GTGATTGGAG ACAGAAGGTC CCGGGAGCAG AAAGCCAAAC 301 AGGAGCGGGA GAAAGAACTG GCAAAAGTCA CTATCAAGAA GGAAGATCTG GAGCTAATAA 361 TGACTGAGAT GGAGATATCT CGAGCAGCAG CAGAACGCAG TTTGCGGGAA CACATGGGCA 421 ACGTGGTAGA GGCGCTTATT GCCCTAACCA ACTGCCCAAC TTTCTTGTAC AAAGTGGTTG 481 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 541 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACG 601 TCCTTCGTGC GATCCATGGA ACACGCGTTA AGTCgacaat caacctctgg attacaaaat 661 ttgtgaaaga tt