Construct: ORF TRCN0000478290
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004801.1_s317c1
- Derived from:
- ccsbBroadEn_08870
- DNA Barcode:
- CCGTCAGGCGTAGGATCTCGATAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RMND5A (64795)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478290
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64795 | RMND5A | required for meiotic nuclea... | NM_022780.4 | 99.9% | 100% | 360G>A |
| 2 | mouse | 68477 | Rmnd5a | required for meiotic nuclea... | NM_001355745.1 | 94.9% | 100% | (many diffs) |
| 3 | mouse | 68477 | Rmnd5a | required for meiotic nuclea... | NM_024288.2 | 94.9% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1239
- ORF length:
- 1173
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tcagtgcgtg acggtggagc gcgagctgga gaaggtgctg cacaagttct 121 caggctacgg gcagctgtgc gagcgcggcc tggaggagct catcgactac accggcggcc 181 tcaagcacga gatcctgcag agccacggcc aagatgctga attatcaggg acactttcac 241 ttgttttgac acagtgctgt aaaagaataa aggatactgt tcaaaaattg gcctccgacc 301 acaaagacat ccacagcagt gtttctcggg ttggaaaagc cattgataag aattttgatt 361 ctgacattag cagtgtggga atagatggct gctggcaggc agacagccaa aggcttctca 421 atgaagtgat ggtggagcac ttctttcgac aaggaatgct ggatgtggct gaggagctct 481 gtcaggaatc tggtctttct gtagacccaa gtcagaagga accatttgtg gagttaaata 541 gaatattaga ggcattaaag gtcagagttc tgagacctgc tctggagtgg gcagtgtcaa 601 accgggaaat gcttatagct caaaacagct ccttggaatt taagctacac agactgtatt 661 ttattagctt gttaatgggt ggaaccacaa atcagcgaga ggcattacaa tatgctaaaa 721 attttcagcc atttgcccta aatcatcaaa aagacattca ggttttgatg ggaagccttg 781 tgtacctgag acaagggatt gagaactcac catatgttca cctacttgat gcaaaccagt 841 gggctgatat ctgtgacatc tttacacggg atgcttgtgc cctcctgggg ctctccgtgg 901 agtcccctct cagtgtcagt ttctcagcag gttgtgtggc gctgccagct ttaattaaca 961 tcaaagccgt gattgaacag aggcagtgta ctggagtttG GAACCAGAAA GATGAATTAC 1021 CTATTGAAGT GGACCTTGGT AAAAAGTGCT GGTATCACTC TATATTTGCC TGCCCCATTC 1081 TTCGTCAGCA AACAACAGAT AACAATCCAC CCATGAAATT GGTCTGTGGT CATATTATAT 1141 CAAGAGATGC CCTGAATAAA ATGTTTAATG GTAGCAAATT AAAATGTCCC TACTGTCCAA 1201 TGGAACAAAG TCCAGGAGAT GCCAAACAGA TATTTTTCTG CCCAACTTTC TTGTACAAAG 1261 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1321 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1381 ACGACCGTCA GGCGTAGGAT CTCGATACAC GCGTTAAGTC gacaatcaac ctctggatta 1441 caaaatttgt gaaagatt