Construct: ORF TRCN0000478291
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003648.1_s317c1
- Derived from:
- ccsbBroadEn_07617
- DNA Barcode:
- GACTAATCTACCGGTGAGCGGTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HMGN4 (10473)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478291
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10473 | HMGN4 | high mobility group nucleos... | NM_006353.3 | 99.6% | 100% | 198G>A |
| 2 | human | 10473 | HMGN4 | high mobility group nucleos... | XM_017010172.2 | 99.6% | 100% | 198G>A |
| 3 | human | 10473 | HMGN4 | high mobility group nucleos... | XM_017010173.2 | 99.6% | 100% | 198G>A |
| 4 | human | 3151 | HMGN2 | high mobility group nucleos... | NM_005517.4 | 85.9% | 86.6% | (many diffs) |
| 5 | mouse | 15331 | Hmgn2 | high mobility group nucleos... | NM_016957.3 | 82.9% | 85.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 360
- ORF length:
- 270
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGCCCAAGAG AAAGGCAAAA GGAGATGCTA 121 AAGGTGATAA AGCAAAGGTG AAGGATGAGC CACAGAGGAG ATCAGCTCGG TTGTCTGCTA 181 AACCAGCTCC TCCAAAACCA GAGCCCAGGC CTAAAAAGGC CTCTGCAAAG AAGGGAGAGA 241 AGCTTCCCAA AGGGAGAAAG GGGAAAGCAG ATGCTGGAAA GGATGGAAAC AACCCTGCAA 301 AAAACCGAGA TGCCTCTACA CTCCAGTCCC AGAAAGCGGA AGGCACTGGG GATGCCAAGT 361 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 421 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 481 TTTATATATC TTGTGGAAAG GACGAGACTA ATCTACCGGT GAGCGGTTGA CGCGTTAAGT 541 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt