Construct: ORF TRCN0000478307
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012211.1_s317c1
- Derived from:
- ccsbBroadEn_00126
- DNA Barcode:
- CCTAAACGTTTAGTCGTAGAACCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATP5F1A (498)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478307
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 498 | ATP5F1A | ATP synthase F1 subunit alpha | NM_001001937.1 | 100% | 100% | |
| 2 | human | 498 | ATP5F1A | ATP synthase F1 subunit alpha | NM_004046.6 | 100% | 100% | |
| 3 | human | 498 | ATP5F1A | ATP synthase F1 subunit alpha | NM_001257334.2 | 96% | 96% | 417_418ins66 |
| 4 | human | 498 | ATP5F1A | ATP synthase F1 subunit alpha | NM_001001935.3 | 90.9% | 90.9% | 0_1ins150 |
| 5 | human | 498 | ATP5F1A | ATP synthase F1 subunit alpha | NM_001257335.1 | 90.9% | 90.9% | 0_1ins150 |
| 6 | human | 498 | ATP5F1A | ATP synthase F1 subunit alpha | XM_017025789.1 | 89.9% | 90.7% | 1579_1718del;1758_1759ins41 |
| 7 | mouse | 11946 | Atp5a1 | ATP synthase, H+ transporti... | NM_007505.2 | 88.3% | 97.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1725
- ORF length:
- 1659
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gtccgtgcgc gttgctgcgg ccgtggtccg cgcccttcct cggcgggccg 121 gactggtctc cagaaatgct ttgggttcat ctttcattgc tgcaaggaac ttccatgcct 181 ctaacactca tcttcaaaag actgggactg ctgagatgtc ctctattctt gaagagcgta 241 ttcttggagc tgatacctct gttgatcttg aagaaactgg gcgtgtctta agtattggtg 301 atggtattgc ccgcgtacat gggctgagga atgttcaagc agaagaaatg gtagagtttt 361 cttcaggctt aaagggtatg tccttgaact tggaacctga caatgttggt gttgtcgtgt 421 ttggaaatga taaactaatt aaggaaggag atatagtgaa gaggacagga gccattgtgg 481 acgttccagt tggtgaggag ctgttgggtc gtgtagttga tgcccttggt aatgctattg 541 atggaaaggg tccaattggt tccaagacgc gtaggcgagt tggtctgaaa gcccccggta 601 tcattcctcg aatttcagtg cgggaaccaa tgcagactgg cattaaggct gtggatagct 661 tggtgccaat tggtcgtggt cagcgtgaac tgattattgg tgaccgacag actgggaaaa 721 cctcaattgc tattgacaca atcattaacc agaaacgttt caatgatgga tctgatgaaa 781 agaagaagct gtactgtatt tatgttgcta ttggtcaaaa gagatccact gttgcccagt 841 tggtgaagag acttacagat gcagatgcca tgaagtacac cattgtggtg tcggctacgg 901 cctcggatgc tgccccactt cagtacctgg ctccttactc tggctgttcc atgggagagt 961 attttagaga caatggcaaa catgctttga tcatctatga cgacttatcc aaacaggctg 1021 ttgcttaccg tcagatgtct ctgttgctcc gccgaccccc tggtcgtgag gcctatcctg 1081 gtgatgtgtt ctacctacac tcccggttgc tggagagagc agccaaaatg aacgatgctt 1141 ttggtggtgg ctccttgact gctttgccag tcatagaaac acaggctggt gatgtgtctg 1201 cttacattcc aacaaatgtc atttccatca ctgacggaca gatcttcttg gaaacagaat 1261 tgttctacaa aggtatccgc cctgcaatta acgttggtct gtctgtatct cgtgtcggat 1321 ccgctgccCA AACCAGGGCT ATGAAGCAGG TAGCAGGTAC CATGAAGCTG GAATTGGCTC 1381 AGTATCGTGA GGTTGCTGCT TTTGCCCAGT TCGGTTCTGA CCTCGATGCT GCCACTCAAC 1441 AACTTTTGAG TCGTGGCGTG CGTCTAACTG AGTTGCTGAA GCAAGGACAG TATTCTCCCA 1501 TGGCTATTGA AGAACAAGTG GCTGTTATCT ATGCGGGTGT AAGGGGATAT CTTGATAAAC 1561 TGGAGCCCAG CAAGATTACA AAGTTTGAGA ATGCTTTCTT GTCTCATGTC GTCAGCCAGC 1621 ACCAAGCCTT GTTGGGCACT ATCAGGGCTG ATGGAAAGAT CTCAGAACAA TCAGATGCAA 1681 AGCTGAAAGA GATTGTAACA AATTTCTTGG CTGGATTTGA AGCTTACCCA ACTTTCTTGT 1741 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1801 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1861 GAAAGGACGA CCTAAACGTT TAGTCGTAGA ACCAACGCGT TAAGTCgaca atcaacctct 1921 ggattacaaa atttgtgaaa gatt